caa a22 4500 445343087 CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2156-8 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2156-8 The potential use of 29kDa protein as a marker of pathogenicity and diagnosis of symptomatic infections with Blastocystis hominis [Elektronische Daten] [Maha Gamra, Hala Elwakil, Hala El Deeb, Khalifa Khalifa, Hala Elhafiz] The present study was performed to characterize the protein profiles of Blastocystis hominis isolates from symptomatic and asymptomatic individuals by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from symptomatic and asymptomatic patients. The presence of immunogenic bands associated with pathogenicity or of diagnostic potentials was also evaluated. The study comprised 80 individuals classified into four groups, 20 each: symptomatic blastocystosis (G1), asymptomatic blastocystosis (G2), other parasitic infections (G3), and healthy control subjects (G4). SDS-PAGE analysis of individual antigens form symptomatic and asymptomatic B. hominis isolates revealed similar and distinctive antigenic bands with significant differences in two high (123.5 and 112.3kDa) and few low molecular weight bands (48.5, 38, 42.3, and 35.5kDa). Immunoblotting was performed using symptomatic and asymptomatic antigen pools with sera of the four studied groups. It was found that anti-B. hominis IgG reacted with nine protein bands ranging from 100 to 18kDa of the symptomatic antigen pool. There was a significant difference between G1 and G2 in the recognition of 64, 56, 38, and 29kDa antigen bands. Also, anti-B. hominis IgG reacted with five protein bands ranging from 56 to 12kDa of asymptomatic antigen pool. There was a significant difference between G1 and G2 in the recognition of 29kDa antigen band. These findings suggest the potential use of the 29-kDa antigen as marker of pathogenicity and implicate its use in the diagnosis and differentiation between symptomatic and asymptomatic blastocystosis. Springer-Verlag, 2010 Gamra Maha Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut Elwakil Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut El Deeb Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut Khalifa Khalifa Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut Elhafiz Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1139-1146 0932-0113 108:5<1139 2011 108 436 https://doi.org/10.1007/s00436-010-2156-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2156-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gamra Maha Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Elwakil Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- El Deeb Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Khalifa Khalifa Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Elhafiz Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1139-1146 0932-0113 108:5<1139 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer