caa a22 4500 445343133 CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2177-3 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2177-3 Molecular cloning and expression of the VK247 circumsporozoite protein for serodiagnosis of variant form Plasmodium vivax [Elektronische Daten] [Tong-Soo Kim, Hyung-Hwan Kim, Sun-Sim Lee, Chang-Mi Oh, Kyung-Mi Choi, Khin Lin, Jung-Yeon Kim, Byoung-Kuk Na, Eun-Taek Han, Youngjoo Sohn, Hyuck Kim, Hyeong-Woo Lee] Plasmodium vivax is classified into two serotypes, VK210 [the dominant form- GDRA(D/A)GQPA repeats] and VK247 [the variant form-ANGA(G/D)(N/D)QPG repeats], based on sequence variation of the repeat region of the circumsporozoite (CS) protein gene. Genomic DNA for the variant CS protein gene was obtained from field isolate strains in Myanmar. The repetitive region has highly 19 immunogenic repeats flanked by non-repeat stretches of amino acids. The sequence including this region (717bp) was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein has a molecular weight of about 50kDa as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Anti-VK247 antibodies were found in malaria patients who have been exposed to variant form of P. vivax in western blot analysis. Therefore, this recombinant protein might be a useful tool in serodiagnosis of malaria patients who have been infected with variant form of P. vivax. Springer-Verlag, 2010 Kim Tong-Soo Department of Parasitology, College of Medicine, Inha University, 405-751, Incheon, South Korea aut Kim Hyung-Hwan Vascular Medicine Research Unit, Brigham and Women's Hospital, Harvard Medical School, 02139, Cambridge, MA, USA aut Lee Sun-Sim Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut Oh Chang-Mi Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut Choi Kyung-Mi Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut Lin Khin Department of Medical Research (Upper Myanmar), Pying Oo Lwin Township, Mandalay, Myanmar aut Kim Jung-Yeon Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut Na Byoung-Kuk Department of Parasitology and Institute of Health Sciences, College of Medicine, Gyeongsang National University, 660-751, Jinju, South Korea aut Han Eun-Taek Department of Parasitology, Kangwon National University, College of Medicine, 200-701, Chuncheon, South Korea aut Sohn Youngjoo Department of Gynecology, College of Oriental Medicine, Sangji University, 220-717, Wonju, South Korea aut Kim Hyuck International Research Center for Bioscience and Biotechnology, Jungwon University, 367-805, Goesan, South Korea aut Lee Hyeong-Woo Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1275-1282 0932-0113 108:5<1275 2011 108 436 https://doi.org/10.1007/s00436-010-2177-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2177-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Tong-Soo Department of Parasitology, College of Medicine, Inha University, 405-751, Incheon, South Korea aut NATIONALLICENCE 700 1- Kim Hyung-Hwan Vascular Medicine Research Unit, Brigham and Women's Hospital, Harvard Medical School, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Lee Sun-Sim Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Oh Chang-Mi Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Choi Kyung-Mi Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Lin Khin Department of Medical Research (Upper Myanmar), Pying Oo Lwin Township, Mandalay, Myanmar aut NATIONALLICENCE 700 1- Kim Jung-Yeon Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Na Byoung-Kuk Department of Parasitology and Institute of Health Sciences, College of Medicine, Gyeongsang National University, 660-751, Jinju, South Korea aut NATIONALLICENCE 700 1- Han Eun-Taek Department of Parasitology, Kangwon National University, College of Medicine, 200-701, Chuncheon, South Korea aut NATIONALLICENCE 700 1- Sohn Youngjoo Department of Gynecology, College of Oriental Medicine, Sangji University, 220-717, Wonju, South Korea aut NATIONALLICENCE 700 1- Kim Hyuck International Research Center for Bioscience and Biotechnology, Jungwon University, 367-805, Goesan, South Korea aut NATIONALLICENCE 700 1- Lee Hyeong-Woo Division of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, 122-701, Seoul, South Korea aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1275-1282 0932-0113 108:5<1275 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer