caa a22 4500 44534315X CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2162-x doi (NATIONALLICENCE)springer-10.1007/s00436-010-2162-x Sensitive and rapid detection of Paragonimus westermani infection in humans and animals by loop-mediated isothermal amplification (LAMP) [Elektronische Daten] [M. Chen, L. Ai, R. Zhang, J. Xia, K. Wang, S. Chen, Y. Zhang, M. Xu, X. Li, X. Zhu, J. Chen] In the present study, a loop-mediated isothermal amplification (LAMP) assay was developed and validated for the detection of Paragonimus westermani adults, metacercariae, and eggs in human and animal samples. The LAMP amplification can be finished in 45min under isothermal condition at 60°C by employing a set of four species-specific primer mixtures and the results can be checked by naked-eye visualization. No amplification products were detected with deoxyribunucleic acid (DNA) of related trematode species including Fasciola hepatica, Fasciola gigantica, Clonorchis sinensis, Opisthorchis viverrini, Schistosoma mansoni, and Schistosoma japonicum. The method was further validated by examining P. westermani DNA in intermediate hosts including freshwater crabs and crayfish, as well as in sputum and pleural fluid samples from patients of paragonimiasis. These results indicated that the LAMP assay was highly specific, sensitive, and rapid, and it was approximately 100 times more sensitive than conventional specific PCR. The LAMP assay established in this study provides a rapid and sensitive tool for the detection of P. westermani DNA in freshwater crabs, crayfish, sputum, and pleural fluid samples, which has important implications for effective control of human paragonimiasis. Springer-Verlag, 2010 Chen M. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut Ai L. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut Zhang R. Shenzhen Center for Disease Control and Prevention, 518020, Shenzhen, Guangdong Province, People's Republic of China aut Xia J. Shenzhen Medical Continuing Education Center, 518020, Shenzhen, Guangdong Province, People's Republic of China aut Wang K. Shenzhen Medical Continuing Education Center, 518020, Shenzhen, Guangdong Province, People's Republic of China aut Chen S. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut Zhang Y. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut Xu M. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu Province, People's Republic of China aut Li X. College of Veterinary Medicine, South China Agricultural University, 510642, Guangzhou, Guangdong Province, People's Republic of China aut Zhu X. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu Province, People's Republic of China aut Chen J. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1193-1198 0932-0113 108:5<1193 2011 108 436 https://doi.org/10.1007/s00436-010-2162-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2162-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen M. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Ai L. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang R. Shenzhen Center for Disease Control and Prevention, 518020, Shenzhen, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Xia J. Shenzhen Medical Continuing Education Center, 518020, Shenzhen, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Wang K. Shenzhen Medical Continuing Education Center, 518020, Shenzhen, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Chen S. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Y. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Xu M. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu Province, People's Republic of China aut NATIONALLICENCE 700 1- Li X. College of Veterinary Medicine, South China Agricultural University, 510642, Guangzhou, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhu X. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu Province, People's Republic of China aut NATIONALLICENCE 700 1- Chen J. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1193-1198 0932-0113 108:5<1193 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer