caa a22 4500 445343222 CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2165-7 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2165-7 Antiplasmodial volatile extracts from Cleistopholis patens Engler & Diels and Uvariastrum pierreanum Engl. (Engl. & Diels) (Annonaceae) growing in Cameroon [Elektronische Daten] [Fabrice Boyom, Vincent Ngouana, Eugenie Kemgne, Paul Zollo, Chantal Menut, Jean Bessiere, Jiri Gut, Philip Rosenthal] In a search for alternative treatment for malaria, plant-derived essential oils extracted from the stem barks and leaves of Cleistopholis patens and Uvariastrum pierreanum (Annonaceae) were evaluated in vitro for antiplasmodial activity against the W2 strain of Plasmodium falciparum. The oils were obtained from 500g each of stem barks and leaves, respectively, by hydrodistillation, using a Clevenger-type apparatus with the following yields: 0.23% and 0.19% for C. patens and 0.1% and 0.3% for U. pierreanum (w/w relative to dried material weight). Analysis of 10% (v/v) oil in hexane by gas chromatography and mass spectrometry identified only terpenoids in the oils, with over 81% sesquiterpene hydrocarbons in C. patens extracts and U. pierreanum stem bark oil, while the leaf oil from the latter species was found to contain a majority of monoterpenes. For C. patens, the major components were α-copaene, δ-cadinene, and germacrene D for the stem bark oil and β-caryophyllene, germacrene D, and germacrene B for the leaf oil. The stem bark oil of U. pierreanum was found to contain mainly β-bisabolene and α-bisabolol, while α- and β-pinenes were more abundant in the leaf extract. Concentrations of oils obtained by diluting 1-mg/mL stock solutions were tested against P. falciparum in culture. The oils were active, with IC50 values of 9.19 and 15.19μg/mL for the stem bark and leaf oils, respectively, of C. patens and 6.08 and 13.96μg/mL, respectively, for those from U. pierreanum. These results indicate that essential oils may offer a promising alternative for the development of new antimalarials. The Author(s), 2010 Boyom Fabrice Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut Ngouana Vincent Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut Kemgne Eugenie Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut Zollo Paul Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut Menut Chantal Laboratoire de Chimie Biomoléculaire, UMR 5032, ENSCM, Montpellier, France aut Bessiere Jean Laboratoire de Phytochimie, ENSCM, Montpellier, France aut Gut Jiri Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA aut Rosenthal Philip Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1211-1217 0932-0113 108:5<1211 2011 108 436 https://doi.org/10.1007/s00436-010-2165-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2165-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boyom Fabrice Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut NATIONALLICENCE 700 1- Ngouana Vincent Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut NATIONALLICENCE 700 1- Kemgne Eugenie Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut NATIONALLICENCE 700 1- Zollo Paul Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon aut NATIONALLICENCE 700 1- Menut Chantal Laboratoire de Chimie Biomoléculaire, UMR 5032, ENSCM, Montpellier, France aut NATIONALLICENCE 700 1- Bessiere Jean Laboratoire de Phytochimie, ENSCM, Montpellier, France aut NATIONALLICENCE 700 1- Gut Jiri Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Rosenthal Philip Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1211-1217 0932-0113 108:5<1211 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer