caa a22 4500 445343230 CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2155-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2155-9 Antibody and cytokine responses to hydatid in experimentally infected Kazakh sheep with hydatidosis resistance haplotype [Elektronische Daten] [Ren-Yan Li, Qiang Peng, Bin Jia, Guo-Qing Shi, Zong-Sheng Zhao, Hong Shen, Hong-Tao Li] Different MHC haplotype of Kazakh sheep has different resistance and susceptibility of hydatidosis. Notably, the MvaIbc-SacIIab-Hin1Iab haplotype of MHC-DRB1 exon two was associated with resistance hydatidosis. In order to analyze the antibody and cytokine responses to hydatidosis in Kazakh sheep with hydatidosis resistance haplotype, eight Kazakh sheep with the haplotype of MvaIbc-SacIIab-Hin1Iab were chosen as the test group, and other eight, which were not associated with hydatidosis resistance or susceptibility, were taken as control. After experimentally infected with hydatid orally, the blood was collected on 0, 7, 14, 30, 45, 60, 75, 90, 105, and 120days. Serum and mRNA level of the cytokines IL-2, IFN-γ, TNF-α, IL-4, and IL-10 were evaluated by ELISA and fluorescence quantitative real-time polymerase chain reaction, respectively. The total white blood cells and leukomonocytes were determined by automation cytoanalyze. The level of IgE, IgG, and IgM were evaluated by ELISA. The results showed that the total white blood cells and leukomonocytes in test group were significantly higher than in control on 7, 45, 90, and 105days post-infection (p.i.). The serum level of IL-2 in test group was significantly higher than in control on 45days p.i., while the difference of IL-2 mRNA expression between test and control group was not significant. The serum level of TNF-α in test group was significantly higher than in control at 90 and 105days p.i., and the TNF-α mRNA in test group was also significantly higher than in control on 90days p.i. The level of IgE, IgG, and IgM in test group was higher than in control, but none was significant. The results suggested that the test group, which was predominant of Th1, could induce the protective immunity, while the control, which was predominant of Th2, could induce the susceptibility to infection of hydatidosis. Springer-Verlag, 2010 Li Ren-Yan College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut Peng Qiang College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut Jia Bin College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut Shi Guo-Qing The Key Sheep Breeding and Reproduction Biotechnology Laboratory, Xinjiang Production and Construction Group, 832003, Shihezi, Sinkiang, China aut Zhao Zong-Sheng College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut Shen Hong College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut Li Hong-Tao College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1131-1137 0932-0113 108:5<1131 2011 108 436 https://doi.org/10.1007/s00436-010-2155-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2155-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Ren-Yan College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut NATIONALLICENCE 700 1- Peng Qiang College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut NATIONALLICENCE 700 1- Jia Bin College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut NATIONALLICENCE 700 1- Shi Guo-Qing The Key Sheep Breeding and Reproduction Biotechnology Laboratory, Xinjiang Production and Construction Group, 832003, Shihezi, Sinkiang, China aut NATIONALLICENCE 700 1- Zhao Zong-Sheng College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut NATIONALLICENCE 700 1- Shen Hong College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut NATIONALLICENCE 700 1- Li Hong-Tao College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Sinkiang, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1131-1137 0932-0113 108:5<1131 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer