caa a22 4500 445343249 CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2179-1 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2179-1 Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis [Elektronische Daten] [Shunyu Li, Jung Shin, Pyo Cho, Tae Kim, Sung-Tae Hong, Sung-Jong Hong] Antigenic proteins from Clonorchis sinensis have been previously purified and evaluated for their antigenicity to enable the serodiagnosis of clonorchiasis. Though they were of high specificity, molecularly defined proteins were reported to be less sensitive as single antigens than crude antigen. To resolve this issue, 11 clones were selected by immunoscreening an adult C. sinensis cDNA library using infected human sera. Mixed antigens were prepared using recombinant proteins of positive clones and investigated for antigenicity by immunoblotting against C. sinensis- and helminth-infected patient sera. A mixed antigen of recombinant 28 and 26kDa glutathion S-transferases (Cs28GST and Cs26GST) produced 76% sensitivity and 95% specificity. Furthermore, a triple mix of recombinant Cs26GST and Cs28GST with vitelline precursor protein pushed up the sensitivity to 87% and maintained specificity at 95%. It is proposed that multiple antigen mixes should be further studied to develop rapid serodiagnostic test kits for the serodiagnosis of human clonorchiasis. Springer-Verlag, 2010 Li Shunyu Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut Shin Jung Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut Cho Pyo Department of Infection Biology, College of Medicine, Wonkwang University, Iksan, 570-749, Jeonbuk, Republic of Korea aut Kim Tae Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut Hong Sung-Tae Department of Parasitology and Tropical Medicine, College of Medicine, Seoul National University, Jongro-gu, 110-799, Seoul, Republic of Korea aut Hong Sung-Jong Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1295-1302 0932-0113 108:5<1295 2011 108 436 https://doi.org/10.1007/s00436-010-2179-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2179-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Shunyu Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Shin Jung Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Cho Pyo Department of Infection Biology, College of Medicine, Wonkwang University, Iksan, 570-749, Jeonbuk, Republic of Korea aut NATIONALLICENCE 700 1- Kim Tae Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Hong Sung-Tae Department of Parasitology and Tropical Medicine, College of Medicine, Seoul National University, Jongro-gu, 110-799, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Hong Sung-Jong Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, 156-756, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1295-1302 0932-0113 108:5<1295 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer