caa a22 4500 44534329X CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2240-0 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2240-0 Development of a PCR approach for differentiation of Alaria spp. mesocercariae [Elektronische Daten] [Katharina Riehn, Ahmad Hamedy, Thomas Alter, Ernst Lücker] To date classification and differentiation of Alaria spp. are based largely on external characteristics and comparative morphology of adult flukes. The accurate differentiation between various Alaria spp. mesocercariae is indeed difficult because there are only few data on morphological and morphometrical features of the parasite's developmental stages. We established a conventional polymerase chain reaction (PCR) for a molecular-based diagnosis of Alaria alata mesocercariae that can aid in their identification. Twenty Alaria spp. mesocercariae specimens were collected from three different wild boars originating from different areas of eastern Germany. DNA from the prepared isolates was extracted, and a primer pair was selected to amplify a 303-bp region of the A. alata genome. The DNA preparations extracted from the field samples as well as A. alata positive controls were successfully amplified and yielded a single sharp band of the expected size. In all samples, molecular identification was consistent with morphological identification. With our new PCR assay, we present the first approach for identification and characterization of A. alata mesocercariae specimens using molecular methods. This practicable and reproducible protocol can be used for both diagnostic and epidemiological purposes. Springer-Verlag, 2011 Riehn Katharina Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany aut Hamedy Ahmad Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany aut Alter Thomas Institute of Food Hygiene, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany aut Lücker Ernst Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1327-1332 0932-0113 108:5<1327 2011 108 436 https://doi.org/10.1007/s00436-010-2240-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2240-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Riehn Katharina Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany aut NATIONALLICENCE 700 1- Hamedy Ahmad Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany aut NATIONALLICENCE 700 1- Alter Thomas Institute of Food Hygiene, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Lücker Ernst Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1327-1332 0932-0113 108:5<1327 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer