caa a22 4500 445343338 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2181-7 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2181-7 In vitro cultivation of Babesia canis canis parasites isolated from dogs in Poland [Elektronische Daten] [Łukasz Adaszek, Stanisław Winiarczyk] The aim of this study was to perform in vitro cultivation of Babesia canis protozoa isolated from dogs with clinical babesiosis. A primary culture initiated in RPMI-1640 medium supplemented with 40% canine serum supported parasite growth in vitro in 5% carbon dioxide in air atmosphere. Subsequent subcultures into HL-1 medium with 40% dog serum or EMEM with 40% foetal bovine serum also supported parasite propagation. The parasites have been continuously cultured through six passages, although the parasitemias are low, ranging from 0.56% to 0.59%. The partial small subunit ribosomal rRNA gene sequence was identical in blood-derived and culture-derived Babesia. The parasites from 17 cultures were classified as EU622792, and from 13 cultures as EU622793. These data show that an efficient in vitro cultivation of B. canis could serve as a starting point to obtain a protozoan antigen used for immunisation of the dogs against piroplasmosis Springer-Verlag, 2010 Adaszek Łukasz Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 30 Głęboka St., 20-612, Lublin, Poland aut Winiarczyk Stanisław Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 30 Głęboka St., 20-612, Lublin, Poland aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1303-1307 0932-0113 108:5<1303 2011 108 436 https://doi.org/10.1007/s00436-010-2181-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2181-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adaszek Łukasz Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 30 Głęboka St., 20-612, Lublin, Poland aut NATIONALLICENCE 700 1- Winiarczyk Stanisław Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, 30 Głęboka St., 20-612, Lublin, Poland aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1303-1307 0932-0113 108:5<1303 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer