caa a22 4500 445343362 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2087-4 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2087-4 Sequencing and analysis of chromosomal extremities of Trypanosoma rangeli in comparison with Trypanosoma cruzi lineages [Elektronische Daten] [Marlene Cabrine-Santos, Luis Ramírez, Eliane Lages-Silva, Bruna de Souza, André Pedrosa] The aim of this study was to investigate the genetic variability of sequences present in the chromosome ends of Trypanosoma rangeli strains defined by the presence (+) or absence (−) of KP1 minicircles, and to compare the mean terminal restriction fragment (TRF) lengths to those of Trypanosoma cruzi populations representative of groups TcI, TcII, TcIV, and TcVI. Southern blots containing RsaI-digested genomic DNA of T. rangeli KP1(+) strains, T. rangeli KP1(−) strains, and T. cruzi strains were probed with the previously described subtelomeric sequences (170bp) of T. rangeli and with telomeric hexamer repeats. Mean TRF length analysis showed that the chromosome ends of T. rangeli are distinctly organized, with TRFs ranging from 1.3 to 9kb for KP1(+) strains and from 0.3 to 5.0kb for KP1(−) strains. In T. cruzi, TRF length ranged from 0.2 to 9kb and no association with the genotype of the parasite could be established. Sequence analysis of the 170-bp amplicons revealed the occurrence of sequence polymorphisms in the subtelomeric region between and within KP1(+) and KP1(−) strains. The GTT triplet was detected in all KP1(+) strains, except for strain Cas4, but not in any of the KP1(−) strains. The dendrogram constructed by alignment of all T. rangeli strains showed the division into two main groups, mainly related to the presence or absence of the KP1 minicircle. In conclusion, the present results extend the genotype differences demonstrated by kDNA and karyotype analysis in T. rangeli to the chromosome ends of the parasite. Springer-Verlag, 2010 Cabrine-Santos Marlene Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut Ramírez Luis Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut Lages-Silva Eliane Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut de Souza Bruna Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut Pedrosa André Discipline of Molecular Biology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 459-466 0932-0113 108:2<459 2011 108 436 https://doi.org/10.1007/s00436-010-2087-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2087-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cabrine-Santos Marlene Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Ramírez Luis Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Lages-Silva Eliane Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- de Souza Bruna Discipline of Parasitology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Pedrosa André Discipline of Molecular Biology, Department of Biological Sciences, Federal University of Triângulo Mineiro, 38025-180, Uberaba, Minas Gerais, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 459-466 0932-0113 108:2<459 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer