caa a22 4500 445343389 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2062-0 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2062-0 Development of a real-time PCR to detect Demodex canis DNA in different tissue samples [Elektronische Daten] [Ivan Ravera, Laura Altet, Olga Francino, Mar Bardagí, Armand Sánchez, Lluís Ferrer] The present study reports the development of a real-time polymerase chain reaction (PCR) to detect Demodex canis DNA on different tissue samples. The technique amplifies a 166bp of D. canis chitin synthase gene (AB 080667) and it has been successfully tested on hairs extracted with their roots and on formalin-fixed paraffin embedded skin biopsies. The real-time PCR amplified on the hairs of all 14 dogs with a firm diagnosis of demodicosis and consistently failed to amplify on negative controls. Eleven of 12 skin biopsies with a morphologic diagnosis of canine demodicosis were also positive. Sampling hairs on two skin points (lateral face and interdigital skin), D. canis DNA was detected on nine of 51 healthy dogs (17.6%) a much higher percentage than previously reported with microscopic studies. Furthermore, it is foreseen that if the number of samples were increased, the percentage of positive dogs would probably also grow. Moreover, in four of the six dogs with demodicosis, the samples taken from non-lesioned skin were positive. This finding, if confirmed in further studies, suggests that demodicosis is a generalized phenomenon in canine skin, due to proliferation of local mite populations, even though macroscopic lesions only appear in certain areas. The real-time PCR technique to detect D. canis DNA described in this work is a useful tool to advance our understanding of canine demodicosis. Springer-Verlag, 2010 Ravera Ivan Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Altet Laura Departament de Ciència Animal i dels Aliments y Servei Veterinari de Genètica Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Francino Olga Departament de Ciència Animal i dels Aliments y Servei Veterinari de Genètica Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Bardagí Mar Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Sánchez Armand Departament de Ciència Animal i dels Aliments y Servei Veterinari de Genètica Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Ferrer Lluís Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 305-308 0932-0113 108:2<305 2011 108 436 https://doi.org/10.1007/s00436-010-2062-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2062-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ravera Ivan Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Altet Laura Departament de Ciència Animal i dels Aliments y Servei Veterinari de Genètica Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Francino Olga Departament de Ciència Animal i dels Aliments y Servei Veterinari de Genètica Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Bardagí Mar Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Sánchez Armand Departament de Ciència Animal i dels Aliments y Servei Veterinari de Genètica Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Ferrer Lluís Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 305-308 0932-0113 108:2<305 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer