caa a22 4500 445343419 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2081-x doi (NATIONALLICENCE)springer-10.1007/s00436-010-2081-x Immune responses to recombinant Brugia malayi pepsin inhibitor homolog (Bm-33) in patients with human lymphatic filariaisis [Elektronische Daten] [N. Krushna, C. Shiny, G. Manokaran, S. Elango, S. Babu, R. Narayanan] Immune responses to recombinant Brugia malayi pepsin inhibitor homolog (rBm-33) were investigated in patients with human lymphatic filariasis (microfilaremics (MF) and chronic pathology (CP)) along with endemic normals (EN). Flow cytometric analysis (24h) revealed CD4+ T cell activation in patients (MF and CP) compared to normals (EN), with increased expression of CD69 and diminished levels of CD62L and CD127. This was associated with an elevated expression of CD154 but not CD28 and CTLA4 in CP patients. However, Bm-33-induced cytokine expression profile (IL-1β, IL-12, IL-8, IFN-γ, IL-10 and TGF-β) did not exhibit any significant difference between normals and patients at the same time point. Although CD4+ T cell activation was observed initially in filarial patients (24h), lymphoproliferation studies (96h) suggested diminished proliferation compared to normals, indicating functional inactivation in the former upon prolonged antigen exposure. This indicates that rBm-33 induces an early T cell activation in MF and CP patients followed by a decreased lymphoproliferation that might contribute to immune suppression in these individuals. Springer-Verlag, 2010 Krushna N. Centre for Biotechnology, Anna University, 600025, Guindy, Chennai, India aut Shiny C. Centre for Biotechnology, Anna University, 600025, Guindy, Chennai, India aut Manokaran G. Apollo Hospital, Greams Road, 600 006, Chennai, India aut Elango S. Department of Public Health and Preventive Medicine, Government of Tamil Nadu, 600 006, Chennai, India aut Babu S. NIH-TRC-ICER SAIC-Frederick Inc., National Cancer Institute at Frederick, Chetpet, 600 003, Chennai, India aut Narayanan R. Centre for Biotechnology, Anna University, 600025, Guindy, Chennai, India aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 407-415 0932-0113 108:2<407 2011 108 436 https://doi.org/10.1007/s00436-010-2081-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2081-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krushna N. Centre for Biotechnology, Anna University, 600025, Guindy, Chennai, India aut NATIONALLICENCE 700 1- Shiny C. Centre for Biotechnology, Anna University, 600025, Guindy, Chennai, India aut NATIONALLICENCE 700 1- Manokaran G. Apollo Hospital, Greams Road, 600 006, Chennai, India aut NATIONALLICENCE 700 1- Elango S. Department of Public Health and Preventive Medicine, Government of Tamil Nadu, 600 006, Chennai, India aut NATIONALLICENCE 700 1- Babu S. NIH-TRC-ICER SAIC-Frederick Inc., National Cancer Institute at Frederick, Chetpet, 600 003, Chennai, India aut NATIONALLICENCE 700 1- Narayanan R. Centre for Biotechnology, Anna University, 600025, Guindy, Chennai, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 407-415 0932-0113 108:2<407 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer