caa a22 4500 445343435 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2055-z doi (NATIONALLICENCE)springer-10.1007/s00436-010-2055-z Identification and characterization of a serine protease inhibitor with two trypsin inhibitor-like domains from the human hookworm Ancylostoma duodenale [Elektronische Daten] [Xian Jin, Li Deng, Hui Li, Zhenlin Zhang, Qingfeng He, Chen Yang, Hanguo Jiang, Xing-Quan Zhu, Lifei Peng] Protease inhibitors play important roles in the parasitic nematodes' survival within their host, in the development and reproduction of the parasites. The present study described the isolation, identification, and characterization of a novel member of the Ascaris family of serine protease inhibitors, designated AduTIL-1, from the human hookworm Ancylostoma duodenale. AduTIL-1 is composed of a signal sequence and two trypsin inhibitor-like (TIL) domains, which showed the highest similarity with OdmCRP, a putative serine protease inhibitor with two TIL domains in Oesophagostomum dentatum. Each TIL domain of the AduTIL-1 was expressed in Escherichia coli, and their inhibitory activities against serine proteases from animals and human were characterized, respectively. Both of the two TIL domains inhibited human neutrophil elastase and pancreatic trypsin, but different in effectiveness. Although the first TIL domain of AduTIL-1 inhibited bovine pancreatic chymotrypsin (Ki = 18.0nM), both of the two domains showed no inhibitory activity against the human pancreatic chymotrypsin. Immunohistochemical studies demonstrated that AduTIL-1 was localized in esophagus, intestine, and cuticular surface of the adult worms. These results suggested that AduTIL-1 may be involved in the survival of A. duodenale in host by targeting related digestive enzymes and neutrophil elastase. Springer-Verlag, 2010 Jin Xian Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut Deng Li Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut Li Hui Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut Zhang Zhenlin Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut He Qingfeng Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut Yang Chen Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut Jiang Hanguo Department of Pathology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut Zhu Xing-Quan State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, 730046, Lanzhou, Gansu Province, People's Republic of China aut Peng Lifei Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 287-295 0932-0113 108:2<287 2011 108 436 https://doi.org/10.1007/s00436-010-2055-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2055-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Xian Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Deng Li Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Li Hui Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zhenlin Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- He Qingfeng Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Yang Chen Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Hanguo Department of Pathology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Xing-Quan State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, 730046, Lanzhou, Gansu Province, People's Republic of China aut NATIONALLICENCE 700 1- Peng Lifei Department of Parasitology and Clinical Parasitology, Guangdong Medical College, 2 Wenming East Street, Xiashan District, 524023, Zhanjiang, Guangdong Province, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 287-295 0932-0113 108:2<287 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer