caa a22 4500 445343443 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2056-y doi (NATIONALLICENCE)springer-10.1007/s00436-010-2056-y In vitro isolation and characterization of biolarvicidal compounds from micropropagated plants of Spilanthes acmella [Elektronische Daten] [Vibha Pandey, Madhu Chopra, Veena Agrawal] Spilanthes acmella (Family: Asteraceae) commonly known as "toothache plant” is known to possess strong insecticidal and larvicidal properties. Experiments have been conducted to isolate and characterise the biolarvicidal compounds from the flower head extract of micropropagated S. acmella plants employing various tools like FT-IR, TLC, CC, NMR. FT-IR spectroscopy of the crude hexane extract sample revealed the presence of amide (secondary metabolite) as functional group in S. acmella flower heads. The crude extract was separated into 85 fractions (100ml each) through silica gel column chromatography using hexane-ethyl acetate mobile phase. All fractions were tested for their larvicidal activity against late III/early IV instar Anopheles stephensi larvae and fraction showing maximum bioefficacy against aforesaid larvae was further resolved into three separate bands on Preparative TLC plate, the respective Rf values being (a) 0.18, (b) 0.23 and (c) 0.27. Based on Proton NMR spectrum of the eluted compounds and their comparison with published results, three different compounds were identified: N-isobutyl-2,6,8-decatrienamide (compound 1), undeca-2E,7Z,9E-trienoic acid isobutylamide (compound 2) from band a and (2E)-N-(2-methylbutyl)-2-undecene-8,10-diynamide (compound 3) from band b. The amount of the compounds obtained were 338mg (compounds 1 and 2) and 188.4mg (compound 3), respectively. This is the first report of biolarvicidal compounds isolation and characterisation from micropropagated S. acmella plants. Springer-Verlag, 2010 Pandey Vibha Department of Botany, University of Delhi, 110007, Delhi, India aut Chopra Madhu B.R. Ambedkar Centre for Biomedical Research, University of Delhi, 110007, Delhi, India aut Agrawal Veena Department of Botany, University of Delhi, 110007, Delhi, India aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 297-304 0932-0113 108:2<297 2011 108 436 https://doi.org/10.1007/s00436-010-2056-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2056-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pandey Vibha Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Chopra Madhu B.R. Ambedkar Centre for Biomedical Research, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Agrawal Veena Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 297-304 0932-0113 108:2<297 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer