caa a22 4500 445343451 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2153-y doi (NATIONALLICENCE)springer-10.1007/s00436-010-2153-y Identification of astigmatid mites using ITS2 and COI regions [Elektronische Daten] [Bin Yang, Junlong Cai, Xunjia Cheng] Identification of astigmatid mites based on their morphological characteristics is difficult because of the similarity of their organs, especially in immature mites. The ribosomal second internal transcribed spacer (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) regions are highly conserved in the eukaryotes and are usually used as barcodes. The ITS2 and COI regions of six species of astigmatid mites (Aleuroglyphus ovatus, Blomia tropicalis, Dermatophagoides farinae, Dermatophagoides pteronyssinus, Euroglyphus maynei, Tyrophagus putrescentiae) were obtained by polymerase chain reaction and sequenced. The lengths of the ITS2 sequences varied from 316 to 488bp, while the COI regions were 377 or 378bp long. Considering the ITS2 genes, the intraspecific genetic distance was in the range of 0.00-0.077844, whereas the interspecific genetic distance was 0.202426-0.912959. The values were 0.000-0.029748 and 0.138403-0.279304 for intra- and interspecific genetic distances when COI genes were used. The phylogenetic trees inferred from the ITS2 and the COI regions, by using maximum parsimony and neighbor-joining methods, were identical to those based on their morphological classification. Thus, the ITS2 and COI regions can be applied as barcodes to identify different species of astigmatid mites. Springer-Verlag, 2010 Yang Bin Department of Microbiology and Parasitology, Shanghai Medical College, Fudan University, No. 138, Yixueyuan Road, 200032, Shanghai, China aut Cai Junlong Department of Microbiology and Parasitology, Shanghai Medical College, Fudan University, No. 138, Yixueyuan Road, 200032, Shanghai, China aut Cheng Xunjia Department of Microbiology and Parasitology, Shanghai Medical College, Fudan University, No. 138, Yixueyuan Road, 200032, Shanghai, China aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 497-503 0932-0113 108:2<497 2011 108 436 https://doi.org/10.1007/s00436-010-2153-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2153-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Bin Department of Microbiology and Parasitology, Shanghai Medical College, Fudan University, No. 138, Yixueyuan Road, 200032, Shanghai, China aut NATIONALLICENCE 700 1- Cai Junlong Department of Microbiology and Parasitology, Shanghai Medical College, Fudan University, No. 138, Yixueyuan Road, 200032, Shanghai, China aut NATIONALLICENCE 700 1- Cheng Xunjia Department of Microbiology and Parasitology, Shanghai Medical College, Fudan University, No. 138, Yixueyuan Road, 200032, Shanghai, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 497-503 0932-0113 108:2<497 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer