caa a22 4500 445343516 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2064-y doi (NATIONALLICENCE)springer-10.1007/s00436-010-2064-y Infection of Anopheles gambiae mosquitoes with entomopathogenic fungi: effect of host age and blood-feeding status [Elektronische Daten] [Ladslaus Mnyone, Matthew Kirby, Monica Mpingwa, Dickson Lwetoijera, Bart Knols, Willem Takken, Constantianus Koenraadt, Tanya Russell] Physiological characteristics of insects can influence their susceptibility to fungal infection of which age and nutritional status are among the most important. An understanding of host-pathogen interaction with respect to these physiological characteristics of the host is essential if we are to develop fungal formulations capable of reducing malaria transmission under field conditions. Here, two independent bioassays were conducted to study the effect of age and blood-feeding status on fungal infection and survival of Anopheles gambiae s.s. Giles. Mosquitoes were exposed to 2 × 1010 conidia m−2 of oil-formulated Metarhizium anisopliae ICIPE-30 and of Beauveria bassiana I93-825, respectively, and their survival was monitored daily. Three age groups of mosquitoes were exposed, 2-4, 5-8, and 9-12days since emergence. Five groups of different feeding status were exposed: non-blood-fed, 3, 12, 36, and 72h post-blood feeding. Fungal infection reduced the survival of mosquitoes regardless of their age and blood-feeding status. Although older mosquitoes died relatively earlier than younger ones, age did not tend to affect mosquito susceptibility to fungal infection. Non-blood-fed mosquitoes were more susceptible to fungus infection compared to all categories of blood-fed mosquitoes, except for those exposed to B. bassiana 72h post-blood feeding. In conclusion, formulations of M. anisopliae and B. bassiana can equally affect mosquitoes of different age classes, with them being relatively more susceptible to fungus infection when non-blood-fed. The Author(s), 2010 Mnyone Ladslaus Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut Kirby Matthew Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut Mpingwa Monica Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut Lwetoijera Dickson Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut Knols Bart Division of Infectious Diseases, Tropical Medicine and AIDS Academic Medical Center, F4-217, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands aut Takken Willem Laboratory of Entomology, Wageningen University and Research Centre, P.O. Box8031, 6700 EH, Wageningen, The Netherlands aut Koenraadt Constantianus Laboratory of Entomology, Wageningen University and Research Centre, P.O. Box8031, 6700 EH, Wageningen, The Netherlands aut Russell Tanya Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 317-322 0932-0113 108:2<317 2011 108 436 https://doi.org/10.1007/s00436-010-2064-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2064-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mnyone Ladslaus Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut NATIONALLICENCE 700 1- Kirby Matthew Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut NATIONALLICENCE 700 1- Mpingwa Monica Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut NATIONALLICENCE 700 1- Lwetoijera Dickson Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut NATIONALLICENCE 700 1- Knols Bart Division of Infectious Diseases, Tropical Medicine and AIDS Academic Medical Center, F4-217, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Takken Willem Laboratory of Entomology, Wageningen University and Research Centre, P.O. Box8031, 6700 EH, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Koenraadt Constantianus Laboratory of Entomology, Wageningen University and Research Centre, P.O. Box8031, 6700 EH, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Russell Tanya Biomedical and Environmental Group, Ifakara Health Institute, P.O. Box53, Off Mlabani Passage, Ifakara, Tanzania aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 317-322 0932-0113 108:2<317 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer