caa a22 4500 445343524 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2085-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2085-6 Entamoeba histolytica calreticulin: an endoplasmic reticulum protein expressed by trophozoites into experimentally induced amoebic liver abscesses [Elektronische Daten] [Enrique González, Maria García de Leon, Isaura Meza, Rodolfo Ocadiz-Delgado, Patricio Gariglio, Angelica Silva-Olivares, Silvia Galindo-Gómez, Mineko Shibayama, Patricia Morán, Alicia Valadez, Angelica Limón, Liliana Rojas, Eric Hernández, René Cerritos, Cecilia Ximenez] Entamoeba histolytica calreticulin (EhCRT) is remarkably immunogenic in humans (90-100% of invasive amoebiasis patients). Nevertheless, the study of calreticulin in this protozoan is still in its early stages. The exact location, biological functions, and its role in pathogenesis are yet to be fully understood. The aim of the present work is to determine the location of EhCRT in virulent trophozoites in vivo and the expression of the Ehcrt gene during the development of experimentally induced amoebic liver abscesses (ALA) in hamsters. Antibodies against recombinant EhCRT were used for the immunolocalization of EhCRT in trophozoites through confocal microscopy; immunohistochemical assays were also performed on tissue sections of ALAs at different times after intrahepatic inoculation. The expression of the Ehcrt gene during the development of ALA was estimated through both in situ RT-PCR and real-time RT-PCR. Confocal assays of virulent trophozoites showed a distribution of EhCRT in the cytoplasmic vesicles of different sizes. Apparently, EhCRT is not exported into the hepatic tissue. Real-time RT-PCR demonstrated an over-expression of the Ehcrt gene at 30min after trophozoite inoculation, reaching a peak at 1-2h; thereafter, the expression fell sharply to its original levels. These results demonstrate for the first time in an in vivo model of ALA, the expression of Ehcrt gene in E. histolytica trophozoites and add evidence that support CRT as a resident protein of the ER in E. histolytica species. The in vivo experiments suggest that CRT may play an important role during the early stages of the host-parasite relationship, when the parasite is adapting to a new environment, although the protein seems to be constitutively synthesized. Moreover, trophozoites apparently do not export EhCRT into the hepatic tissue in ALA. Springer-Verlag, 2010 González Enrique Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut García de Leon Maria Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Meza Isaura Depto. de Biomedicina Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut Ocadiz-Delgado Rodolfo Depto. de Genética y Biología Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut Gariglio Patricio Depto. de Genética y Biología Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut Silva-Olivares Angelica Depto. de Infectómica y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut Galindo-Gómez Silvia Depto. de Infectómica y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut Shibayama Mineko Depto. de Infectómica y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut Morán Patricia Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Valadez Alicia Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Limón Angelica Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Rojas Liliana Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Hernández Eric Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Cerritos René Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Ximenez Cecilia Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 439-449 0932-0113 108:2<439 2011 108 436 https://doi.org/10.1007/s00436-010-2085-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2085-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- González Enrique Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- García de Leon Maria Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- Meza Isaura Depto. de Biomedicina Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut NATIONALLICENCE 700 1- Ocadiz-Delgado Rodolfo Depto. de Genética y Biología Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut NATIONALLICENCE 700 1- Gariglio Patricio Depto. de Genética y Biología Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut NATIONALLICENCE 700 1- Silva-Olivares Angelica Depto. de Infectómica y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut NATIONALLICENCE 700 1- Galindo-Gómez Silvia Depto. de Infectómica y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut NATIONALLICENCE 700 1- Shibayama Mineko Depto. de Infectómica y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360, México, D.F., Mexico aut NATIONALLICENCE 700 1- Morán Patricia Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- Valadez Alicia Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- Limón Angelica Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- Rojas Liliana Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- Hernández Eric Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- Cerritos René Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 700 1- Ximenez Cecilia Depto. de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis No 148, Col. Doctores, CP 06726, México, D.F., Mexico aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 439-449 0932-0113 108:2<439 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer