caa a22 4500 445343583 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2082-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2082-9 prag01 , a novel deltamethrin-resistance-associated gene from Culex pipiens pallens [Elektronische Daten] [Jin Zhang, Mifang Yang, Weijie Wang, Haibo Sun, Yang Xu, Lei Ma, Yan Sun, Changliang Zhu] The prag01 gene (GenBank accession no. EU073017) was cloned from Culex pipiens pallens. An open reading frame of 270bp was found to encode a putative 89-amino-acid protein which has the highest homology with Culex quinquefasciatus and Anopheles funestus. Real-time quantitative PCR analysis demonstrated that the transcription level of prag01 gene in deltamethrin-resistant strain was 1.65-fold higher than in deltamethrin-susceptible strain of C. pipiens pallens. Overexpression of prag01 gene in the mosquito C6/36 cells showed better prolification than the cells with empty vector when treated by deltamethrin. Our data for the first time approved that prag01 gene might play some role in the development of deltamethrin resistance in C. pipiens pallens. Springer-Verlag, 2010 Zhang Jin Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Yang Mifang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Wang Weijie Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Sun Haibo Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Xu Yang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Ma Lei Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Sun Yan Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Zhu Changliang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 417-423 0932-0113 108:2<417 2011 108 436 https://doi.org/10.1007/s00436-010-2082-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2082-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Jin Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Mifang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Weijie Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Sun Haibo Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Xu Yang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Ma Lei Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Sun Yan Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Changliang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 417-423 0932-0113 108:2<417 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer