caa a22 4500 445343591 CHVBK 20180317142821.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2084-7 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2084-7 Schistosoma japonicum -infected hamsters ( Mesocricetus auratus ) used as a model in experimental chemotherapy with praziquantel, artemether, and OZ compounds [Elektronische Daten] [Shu-hua Xiao, Jing-yan Mei, Pei-ying Jiao] The purpose of the study is to better understand the antischistosomal properties of artemether, praziquantel, and ozonide (OZ) compounds (synthetic trioxolanes, secondary ozonides) in hamster (Mesocricetus auratus) model. A total of 230 male hamsters infected each with 100 Schistosoma japonicum cercariae were used in the study. Groups of five to ten hamsters were treated orally with artemether, praziquantel, and OZ78 or OZ277 7-35days post-infection at single doses of 50, 100, 150, or 200mg/kg. Untreated but infected hamsters in each batch of test served as the control. All treated hamsters were sacrificed 4weeks post-treatment for collection of residual worms using perfusion technique. Nonparametric method (Mann-Whitney test) was used to analyze the data. In groups of five hamsters treated with artemether 7, 14, 21, 28, and 35days post-infection at single doses of 150 and 200mg/kg, the difference of mean worm burden between each treated group and control group was statistically significant (P < 0.01). Apart from individual group, no difference in mean worm burden between each two groups of them was seen (P > 0.05). Further test with various single doses of 50-200mg/kg confirmed the similar susceptibility of 7-day-old juvenile and 35-day-old adult schistosomes to artemether. After administration of praziquantel 100mg/kg to groups of five hamsters 7, 21, and 35days post-infection, higher worm burden reduction of 95.5% was seen in the group with 35-day-old adult schistosomes while in the groups with 7- and 21-day-old juvenile schistosomes, poor efficacy was seen with mean worm burden reductions of 36.6% and 35.6%. In the same batch of hamster treated with praziquantel 200mg/kg, the moderate effect of the drug against 7- and 21-day-old worms was seen, but their mean worm burden was significantly higher than that of the group with adult schistosomes. In comparison of artemether and praziquantel against various stages of schistosomes, the results further demonstrated that artemether possessed similar effect against juvenile and adult schistosomes in hamsters, while praziquantel was more effective against adult schistosomes than the juvenile ones in the same host. Finally, after administration of OZ78 and OZ277 to the groups of four to six hamsters with 14- and 35-day-old schistosomes at a single dose of 200mg/kg, promising effect against juvenile and adult schistosome was observed with the mean worm burden and female worm burden reductions of 69.6-94.2% and 64.2-100% as well as 73.3-80.7% and 68.3-81.1%, respectively. The results indicate that in hamster model, praziquantel exhibits higher effect against adult schistosomes than the juvenile ones, while artemether and OZ compound display similar effect against both juvenile and adult schistosomes. Springer-Verlag, 2010 Xiao Shu-hua National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, 200025, Shanghai, People's Republic of China aut Mei Jing-yan National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, 200025, Shanghai, People's Republic of China aut Jiao Pei-ying National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, 200025, Shanghai, People's Republic of China aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 431-437 0932-0113 108:2<431 2011 108 436 https://doi.org/10.1007/s00436-010-2084-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2084-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiao Shu-hua National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Mei Jing-yan National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Jiao Pei-ying National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 431-437 0932-0113 108:2<431 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer