caa a22 4500 445343656 CHVBK 20180317142822.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00436-010-2074-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2074-9 Comparative analysis of the diagnostic performance of crude sheep hydatid cyst fluid, purified antigen B and its subunit (12Kda), assessed by ELISA, in the diagnosis of human cystic echinococcosis [Elektronische Daten] [Gihan Tawfeek, Hala Elwakil, Laila El-hoseiny, Hala Thabet, Rania Sarhan, Nabil Awad, Wagida Anwar] The diagnosis of patients with cystic echinococcosis (CE) by means of serology has a limited support in clinical practice due to cross-reactivity with other helminthes leading to overestimation of the parasite's true prevalence. A wealth of reports on the diagnostic performance of antigen B (AgB) has been produced. This study was designed to comparatively assess the diagnostic efficacy of crude sheep hydatid cyst fluid (HCF), AgB and its subunit (12KDa) to detect IgG or IgG4 antibodies in CE patients' sera using enzyme-linked immunosorbent assay (ELISA).The best diagnostic performance was obtained with anti-HCF IgG ELISA which gave 92.4% sensitivity and 92.6% specificity. Despite the low sensitivity of anti AgB IgG ELISA (84%), it gave the best specificity (94.4%) with less cross-reaction with sera of subjects infected with other parasites. In conclusion, it is recommended to use anti-HCF IgG ELISA for initial screening in large seroprevalence studies. Further analysis of positive serum samples with anti AgB IgG ELISA would allow the confirmation of true positives. Specific IgG4 ELISA may represent a complementary assay, useful as secondary confirmatory tests for patients with suspected CE and negative for total IgG ELISA. Springer-Verlag, 2010 Tawfeek Gihan Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut Elwakil Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut El-hoseiny Laila Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut Thabet Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut Sarhan Rania Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut Awad Nabil Faculty of Biotechnology, Misr University for Science and Technology (MUST), 6th of October, Egypt aut Anwar Wagida Department of Public Health, Faculty of Medicine, Ain Shams University, Cairo, Egypt aut Parasitology Research Springer-Verlag 108/2(2011-02-01), 371-376 0932-0113 108:2<371 2011 108 436 https://doi.org/10.1007/s00436-010-2074-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2074-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tawfeek Gihan Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Elwakil Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- El-hoseiny Laila Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Thabet Hala Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Sarhan Rania Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St, Abbassia, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Awad Nabil Faculty of Biotechnology, Misr University for Science and Technology (MUST), 6th of October, Egypt aut NATIONALLICENCE 700 1- Anwar Wagida Department of Public Health, Faculty of Medicine, Ain Shams University, Cairo, Egypt aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/2(2011-02-01), 371-376 0932-0113 108:2<371 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer