caa a22 4500 445343915 CHVBK 20180317142823.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00406-011-0197-3 doi (NATIONALLICENCE)springer-10.1007/s00406-011-0197-3 Protective effects of haloperidol and clozapine on energy-deprived OLN-93 oligodendrocytes [Elektronische Daten] [Johann Steiner, Zoltán Sarnyai, Sabine Westphal, Tomasz Gos, Hans-Gert Bernstein, Bernhard Bogerts, Gerburg Keilhoff] Magnetic resonance imaging and postmortem studies on schizophrenia provided evidence for compromised myelin integrity and reduced numbers of oligodendrocytes, which may worsen during the disease course. However, it is not clear whether these findings result from disease-inherent oligodendrocyte degeneration or side effects of antipsychotic treatment. Therefore, effects of haloperidol and clozapine on the viability and apoptosis of immature oligodendrocytes (OLN-93 cells, immunopositive for NG2, Olig1, Olig2) have been evaluated in the present study by labeling with propidium iodide and a caspase 3 assay. Given the indications for impaired cerebral energy supply in schizophrenia, a serum and glucose deprivation (SGD) model was chosen in comparison with the basal condition (BC). SGD led to increased necrotic and apoptotic cell death. Haloperidol and clozapine were partially protective in this model and reduced the percentage of propidium iodide-positive cells, while caspase 3 activity was not altered. No significant drug effects were observed under BC. The observed protective effects of haloperidol and clozapine on energy-deprived OLN-93 oligodendrocytes suggest that previously reported reductions in oligodendrocyte density in schizophrenia are rather disease related than a side effect of medication. A new mechanism of antipsychotic action is suggested, which may help to establish new oligodendrocyte-directed therapies of schizophrenia. Springer-Verlag, 2011 Schizophrenia nationallicence Oligodendrocytes nationallicence OLN-93 cells nationallicence Clozapine nationallicence Haloperidol nationallicence Steiner Johann Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Sarnyai Zoltán Department of Pharmacology, University of Cambridge, Cambridge, UK aut Westphal Sabine Institute of Clinical Chemistry and Pathobiochemistry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Gos Tomasz Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Bernstein Hans-Gert Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Bogerts Bernhard Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Keilhoff Gerburg Institute of Biochemistry and Cell Biology, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut European Archives of Psychiatry and Clinical Neuroscience Springer-Verlag 261/7(2011-10-01), 477-482 0940-1334 261:7<477 2011 261 406 https://doi.org/10.1007/s00406-011-0197-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00406-011-0197-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steiner Johann Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Sarnyai Zoltán Department of Pharmacology, University of Cambridge, Cambridge, UK aut NATIONALLICENCE 700 1- Westphal Sabine Institute of Clinical Chemistry and Pathobiochemistry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Gos Tomasz Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Bernstein Hans-Gert Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Bogerts Bernhard Department of Psychiatry, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Keilhoff Gerburg Institute of Biochemistry and Cell Biology, University of Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 773 0- European Archives of Psychiatry and Clinical Neuroscience Springer-Verlag 261/7(2011-10-01), 477-482 0940-1334 261:7<477 2011 261 406 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer