caa a22 4500 445344636 CHVBK 20180317142825.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00406-010-0129-7 doi (NATIONALLICENCE)springer-10.1007/s00406-010-0129-7 Postmortem proteomic analysis in human amygdala of drug addicts: possible impact of tubulin on drug-abusing behavior [Elektronische Daten] [P. Zill, V. Vielsmeier, A. Büttner, W. Eisenmenger, F. Siedler, B. Scheffer, H.-J. Möller, B. Bondy] Besides the ventral tegmental area and the nucleus accumbens as the most investigated brain reward structures, several reports about the relation between volume and activity of the amygdala and drug-seeking behavior have emphasized the central role of the amygdala in the etiology of addiction. Considering its proposed important role and the limited number of human protein expression studies with amygdala in drug addiction, we performed a human postmortem proteomic analysis of amygdala tissue obtained from 8 opiate addicts and 7 control individuals. Results were validated by Western blot in an independent postmortem replication sample from 12 opiate addicts compared to 12 controls and 12 suicide victims, as a second "control sample”. Applying 2D-electrophoresis and MALDI-TOF-MS analysis, we detected alterations of beta-tubulin expression and decreased levels of the heat-shock protein HSP60 in drug addicts. Western blot analysis in the additional sample demonstrated significantly increased alpha- and beta-tubulin concentrations in the amygdala of drug abusers versus controls (P=0.021, 0.029) and to suicide victims (P=0.006, 0.002). Our results suggest that cytoskeletal alterations in the amygdala determined by tubulin seem to be involved in the pathophysiology of drug addiction, probably via a relation to neurotransmission and cellular signaling. Moreover, the loss of neuroprotection against stressors by chaperons as HSP60 might also contribute to structural alteration in the brain of drug addicts. Although further studies have to confirm our results, this might be a possible pathway that may increase our understanding of drug addiction. Springer-Verlag, 2010 Drug nationallicence Addiction nationallicence Amygdala nationallicence Human nationallicence Proteomics nationallicence Tubulin nationallicence Zill P. Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut Vielsmeier V. Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut Büttner A. Institute for Legal Medicine, Ludwig-Maximilians-University Munich, Frauenlobstrasse 7, 80337, Munich, Germany aut Eisenmenger W. Institute for Legal Medicine, Ludwig-Maximilians-University Munich, Frauenlobstrasse 7, 80337, Munich, Germany aut Siedler F. Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany aut Scheffer B. Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany aut Möller H.-J Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut Bondy B. Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut European Archives of Psychiatry and Clinical Neuroscience Springer-Verlag 261/2(2011-03-01), 121-131 0940-1334 261:2<121 2011 261 406 https://doi.org/10.1007/s00406-010-0129-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00406-010-0129-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zill P. Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut NATIONALLICENCE 700 1- Vielsmeier V. Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut NATIONALLICENCE 700 1- Büttner A. Institute for Legal Medicine, Ludwig-Maximilians-University Munich, Frauenlobstrasse 7, 80337, Munich, Germany aut NATIONALLICENCE 700 1- Eisenmenger W. Institute for Legal Medicine, Ludwig-Maximilians-University Munich, Frauenlobstrasse 7, 80337, Munich, Germany aut NATIONALLICENCE 700 1- Siedler F. Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany aut NATIONALLICENCE 700 1- Scheffer B. Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany aut NATIONALLICENCE 700 1- Möller H.-J Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut NATIONALLICENCE 700 1- Bondy B. Department of Psychiatry, Division of Psychiatric Genetics and Neurochemistry, Ludwig-Maximilians-University Munich, Nussbaumstrasse 7, 80336, Munich, Germany aut NATIONALLICENCE 773 0- European Archives of Psychiatry and Clinical Neuroscience Springer-Verlag 261/2(2011-03-01), 121-131 0940-1334 261:2<121 2011 261 406 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer