caa a22 4500 445347619 CHVBK 20180317142834.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00056-011-0050-3 doi (NATIONALLICENCE)springer-10.1007/s00056-011-0050-3 Expression of osteoprotegerin and receptor activator of nuclear factor κB ligand in root resorption induced by heavy force in rats [Elektronische Daten] [J.P. Zhou, G. Feng, W.W, Zhou, A.S. Ren, Y. Wu, D.M. Zhang, H.W. Dai] Die Expression von Osteoprotegerin und des Rezeptor-Aktivators des Nuklearfaktor-κ-B-Liganden bei der durch starke Krafteinwirkung induzierten Wurzelresorption bei Ratten Objective: The aim of this study was to investigate the expression patterns of osteoprotegerin (OPG) and the receptor activator of nuclear factor κB ligand (RANKL) in root resorption during orthodontic tooth movement. Material and methods: Forty 12-week-old male SD rats were used with the right maxillary side as the experimental group and the left maxillary side as the control group. After 1N (100g) force was loaded on the right maxillary first molar, the rats were sacrificed on days 0, 1, 4, 8, and 12. Mesial root resorption of the first molar, the number of odontoclasts and osteoclasts, and OPG and RANKL mRNA expression were determined by hematoxylin-eosin and scanning electron microscopy, tartrate-resistant acid phosphate staining, and in situ hybridization, respectively. Results: Serious root resorption was apparent on the pressure side of the mesial root of the right maxillary first molar on days 8 and 12. The number of odontoclasts in the cementum lacuna was elevated on days 8 and 12. OPG expression rose significantly on the tensile side, while RANKL expression increased on the pressure side. The mRNA level of RANKL was significantly elevated on days 4, 8, and 12. Moreover, the RANKL/OPG mRNA ratio was increased on the pressure side, but decreased on the tensile side. Conclusion: Changes in the expression of RANKL mRNA and the RANKL/OPG mRNA ratio are accompanied by a parallel alteration in the number of odontoclasts and tooth resorption, suggesting crucial involvement of RANKL and OPG in tooth resorption. Urban &amp; Vogel, Muenchen, 2011 Tooth movement nationallicence Root resorption nationallicence Osteoprotegerin nationallicence Receptor activator of nuclear factor-κ B ligand nationallicence Zahnbewegung nationallicence Wurzelresorption nationallicence Osteoprotegerin nationallicence Rezeptor-Aktivator des Nuklearfaktor-κ-B-Liganden nationallicence Zhou J.P. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut Feng G. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut Zhou W.W, Department of Prosthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China aut Ren A.S. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut Wu Y. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut Zhang D.M. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut Dai H.W. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie Springer-Verlag 72/6(2011-11-01), 457-468 1434-5293 72:6<457 2011 72 56 https://doi.org/10.1007/s00056-011-0050-3 text/html Onlinezugriff via DOI 1 article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00056-011-0050-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou J.P. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut NATIONALLICENCE 700 1- Feng G. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut NATIONALLICENCE 700 1- Zhou W.W. Department of Prosthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China aut NATIONALLICENCE 700 1- Ren A.S. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut NATIONALLICENCE 700 1- Wu Y. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut NATIONALLICENCE 700 1- Zhang D.M. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut NATIONALLICENCE 700 1- Dai H.W. Department of Orthodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, NO.7 Shangqingsi Road, Yuzhong District, 400015, Chongqing, China aut NATIONALLICENCE 773 0- Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie Springer-Verlag 72/6(2011-11-01), 457-468 1434-5293 72:6<457 2011 72 56 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer