caa a22 4500 445353457 CHVBK 20180317142852.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00221-011-2702-3 doi (NATIONALLICENCE)springer-10.1007/s00221-011-2702-3 Effects of hyperbaric oxygen on the expression of claudins after cerebral ischemia-reperfusion in rats [Elektronische Daten] [Hong Zhao, Qianru Zhang, Yixue Xue, Xuexin Chen, Randy Haun] The malfunction of tight junctions (TJs) between endothelial cells in the blood brain barrier (BBB) is the pathophysiological basis for cerebral ischemia-reperfusion (IR) injury. Claudins, major molecular elements of the TJs, play a key role in the paracellular permeability of the BBB. Although several studies have demonstrated the impact of hyperbaric oxygenation (HBO) on boosting oxygen supply and reducing infarct size, its effect and underlying mechanism on the integrity of the BBB is unknown. To study the function of HBO on claudins and the permeability of the BBB, we replicated the animal model of local cerebral IR. Using Evans blue dye, permeability of the BBB was examined. Transmission electron microscopy (TEM), immunohistochemistry, western blot, and gelatin zymography were used to detect the integrity of the BBB, the expression of claudin-1 and claudin-5, and the activity of matrix metalloproteinases (MMPs) in brain microvessel endothelium. Our data indicate that compared with the sham-operated group, IR increased permeability of the BBB to Evans blue dye (P<0.01), peaking at 4h. The BBB ultrastructure was disrupted and the expression of claudin-5 and claudin-1 decreased (P<0.01) in the 4 and 72h IR group, respectively. Increased claudin-5 and claudin-1 expression and decreased permeability of the BBB were observed in the HBO+IR group (P<0.01) via the suppression of MMP-2 and MMP-9, respectively. Our study provides direct evidence that HBO decreases the permeability of the BBB by reducing the enzymatic activity of MMPs and augmenting the expression of claudins at different stages in cerebral IR injury. Springer-Verlag, 2011 Blood brain barrier nationallicence Ischemia-reperfusion nationallicence Hyperbaric oxygen nationallicence Claudin nationallicence AJ : Adherens junction nationallicence BBB : Blood brain barrier nationallicence CCA : Common carotid artery nationallicence EB : Evans blue nationallicence ECA : External carotid artery nationallicence HBO : Hyperbaric oxygenation nationallicence HRP : Horseradish peroxidase nationallicence ICA : Internal carotid artery nationallicence IR : Ischemia-reperfusion nationallicence MCAO : Middle cerebral artery occlusion nationallicence MMP : Matrix metalloproteinase nationallicence RT-PCR : Reverse transcription polymerase chain reaction nationallicence SABC : Streptoavidin-biotin complex nationallicence TJ : Tight junction nationallicence Zhao Hong Department of Experimental Center of Functional Subjects, College of Basic Medicine, China Medical University, 110001, Shenyang, China aut Zhang Qianru Department of Experimental Center of Functional Subjects, College of Basic Medicine, China Medical University, 110001, Shenyang, China aut Xue Yixue Department of Neurobiology, College of Basic Medicine, China Medical University, 110001, Shenyang, China aut Chen Xuexin Department of Hyperbaric Oxygen, The 1st Affiliated Hospital of China Medical University, 110001, Shenyang, China aut Haun Randy Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences, 72205-7199, Little Rock, AR, USA aut Experimental Brain Research Springer-Verlag 212/1(2011-07-01), 109-117 0014-4819 212:1<109 2011 212 221 https://doi.org/10.1007/s00221-011-2702-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00221-011-2702-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Hong Department of Experimental Center of Functional Subjects, College of Basic Medicine, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Zhang Qianru Department of Experimental Center of Functional Subjects, College of Basic Medicine, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Xue Yixue Department of Neurobiology, College of Basic Medicine, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Chen Xuexin Department of Hyperbaric Oxygen, The 1st Affiliated Hospital of China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Haun Randy Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences, 72205-7199, Little Rock, AR, USA aut NATIONALLICENCE 773 0- Experimental Brain Research Springer-Verlag 212/1(2011-07-01), 109-117 0014-4819 212:1<109 2011 212 221 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer