caa a22 4500 445354135 CHVBK 20180317142854.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00221-010-2459-0 doi (NATIONALLICENCE)springer-10.1007/s00221-010-2459-0 Intracranial landmarks and other techniques to further improve the precision of stereotaxic tracer injections [Elektronische Daten] [Zsolt Kovács, Miklós Palkovits] This study is meant to combine traditional aspects of tracer microinjections using bone landmarks like bregma and lambda with novel procedures in which specific parts of the brain can serve as reference points. For telencephalic and diencephalic injections, the brain surface, the interhemispheric groove and the straight sinus can be used as absolute zero points for dorso-ventral, medio-lateral and rostro-caudal coordinates, respectively. In case of brainstem targets, the surface of the rhomboid fossa, the posterior spinal artery and the obex could serve as reference points along the above-mentioned coordinates. The application of high-precision stereotaxic measurements based on intracranial landmarks and sophisticated surgical procedures can yield well-targeted, small and well circumscribed injection sites that make possible the mapping of discrete nuclear subdivisions or delicate nuclei in the brain. Springer-Verlag, 2010 Cranial landmarks nationallicence Intracranial landmarks nationallicence Straight sinus nationallicence Obex nationallicence Virus microinjections nationallicence Brain mapping nationallicence Interhemispheric groove/fissure nationallicence Kovács Zsolt Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Semmelweis University, Tüzoltó-utca 58, 1094, Budapest, Hungary aut Palkovits Miklós Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Semmelweis University, Tüzoltó-utca 58, 1094, Budapest, Hungary aut Experimental Brain Research Springer-Verlag 208/1(2011-01-01), 51-60 0014-4819 208:1<51 2011 208 221 https://doi.org/10.1007/s00221-010-2459-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00221-010-2459-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kovács Zsolt Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Semmelweis University, Tüzoltó-utca 58, 1094, Budapest, Hungary aut NATIONALLICENCE 700 1- Palkovits Miklós Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Semmelweis University, Tüzoltó-utca 58, 1094, Budapest, Hungary aut NATIONALLICENCE 773 0- Experimental Brain Research Springer-Verlag 208/1(2011-01-01), 51-60 0014-4819 208:1<51 2011 208 221 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer