caa a22 4500 445354607 CHVBK 20180317142855.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10238-010-0105-6 doi (NATIONALLICENCE)springer-10.1007/s10238-010-0105-6 Effect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model [Elektronische Daten] [Dandan Wang, Huayong Zhang, Jun Liang, Zhifeng Gu, Xiaolei Ma, Jing Huang, Jing Lin, Yayi Hou, Liwei Lu, Lingyun Sun] Primary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. After 6weeks of MSC infusion, serum aminotransferase and autoimmune antibodies declined, and histological examination by hematoxylin and eosin staining showed significant amelioration of monocytes infiltration around bile ducts of mice treated with BM-MSC. Interestingly, allogeneic BM-MSC transplantation markedly increased CD4+Foxp3+ regulatory T cells in peripheral blood as well as in lymph nodes when analyzed by flow cytometry. Further examination showed serum TGF-β1 increased but IFN-γ decreased significantly in PBC mice treated with MSC, while with no obvious change in IL-10 expression. Our results for the first time suggested that BM-MSC transplantation could regulate systemic immune response and enhance recovery in liver inflammation of PBC mice, raising the possibility for clinical application of allogeneic MSC in treatment of early-stage PBC patients. Springer-Verlag, 2010 Bone marrow nationallicence MSC nationallicence PBC nationallicence Regulatory T cells nationallicence Cytokine nationallicence Wang Dandan Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Zhang Huayong Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Liang Jun Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Gu Zhifeng Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Ma Xiaolei Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Huang Jing Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Lin Jing Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Hou Yayi Immunology Laboratory, Nanjing University Medical School, Nanjing, China aut Lu Liwei Department of Pathology and Center of Infection and Immunology, The University of Hong Kong, Hong Kong, China aut Sun Lingyun Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut Clinical and Experimental Medicine Springer Milan 11/1(2011-03-01), 25-32 1591-8890 11:1<25 2011 11 10238 https://doi.org/10.1007/s10238-010-0105-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-010-0105-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Dandan Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Zhang Huayong Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Liang Jun Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Gu Zhifeng Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Ma Xiaolei Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Huang Jing Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Lin Jing Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Hou Yayi Immunology Laboratory, Nanjing University Medical School, Nanjing, China aut NATIONALLICENCE 700 1- Lu Liwei Department of Pathology and Center of Infection and Immunology, The University of Hong Kong, Hong Kong, China aut NATIONALLICENCE 700 1- Sun Lingyun Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, 210008, Nanjing, Jiangsu, China aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/1(2011-03-01), 25-32 1591-8890 11:1<25 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer