caa a22 4500 445354658 CHVBK 20180317142855.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10238-010-0106-5 doi (NATIONALLICENCE)springer-10.1007/s10238-010-0106-5 Establishment of airway eosinophilic bronchitis mouse model without hyperresponsiveness by ovalbumin [Elektronische Daten] [Liyan Chen, Kefang Lai, Jiaxing Xie, Nanshan Zhong] Eosinophilic bronchitis (EB) is a useful tool for studying airway hyperresponsiveness (AHR), but an exact EB animal model is lacking. Our objective was to establish an EB mouse model using ovalbumin (OVA). Mice were divided into asthma, normal saline (NS) control and three model groups. Asthma mice were challenged intranasally with 200μg OVA. Model groups were challenged with one of the three OVA doses (10, 20 or 100μg), and NS control mice received normal saline. Changes in lung resistance (RL), serum OVA-specific immunoglobulin-E (IgE) and differential inflammatory cell counts in bronchoalveolar lavage fluid (BALF) were determined after exposure to increasing doses of methacholine (MCh). Lung histological sections were examined for inflammatory infiltration. RL in the 10-μg OVA-challenged model group was not significantly different compared with the NS group at any MCh concentration but was significantly different compared with the asthma group (P<0.01). RL in the other two model groups was intermediate between the asthma and NS groups. Serum OVA-specific IgE and eosinophils in BALF were increased significantly in all model and asthma groups compared with the NS group, but no significant differences were observed between model and asthma groups. Inflammatory cells were seen around bronchioles and capillaries in model and asthma groups but not the NS group. A mouse model of EB without AHR can be established by 10μg OVA challenge and provides a useful tool for studying AHR mechanisms. Springer-Verlag, 2010 Airway eosinophilia nationallicence Asthma nationallicence Mouse model nationallicence Airway responsiveness nationallicence Eosinophilic bronchitis nationallicence Chen Liyan State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut Lai Kefang State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut Xie Jiaxing State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut Zhong Nanshan State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut Clinical and Experimental Medicine Springer Milan 11/1(2011-03-01), 19-24 1591-8890 11:1<19 2011 11 10238 https://doi.org/10.1007/s10238-010-0106-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-010-0106-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Liyan State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Lai Kefang State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Xie Jiaxing State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Zhong Nanshan State Key Laboratory of Respiratory Diseases, Guangzhou Medical College, 151 Yanjiang Road, 510120, Guangzhou, Guangdong, China aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/1(2011-03-01), 19-24 1591-8890 11:1<19 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer