caa a22 4500 445354739 CHVBK 20180317142856.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10238-010-0118-1 doi (NATIONALLICENCE)springer-10.1007/s10238-010-0118-1 Lipopolysaccharide (LPS) of Helicobacter modulates cellular DNA repair systems in intestinal cells [Elektronische Daten] [Pasqua Cavallo, Antonia Cianciulli, Vincenzo Mitolo, Maria Panaro] The epithelium of the intestinal tract is exposed to a variety of genotoxic agents, both exogenous and endogenous, that can injure nuclear and mitochondrial DNA. DNA damage can be repaired by a series of DNA repair enzymes, while defects in this system will make these cells once more susceptible to malignant transformation or cell death. Recent studies suggest that intestinal bacteria may contribute to induce inflammation in individuals afflicted by inflammatory bowel disease (IBD), increasing the risk of developing colon cancer. Accumulating evidence suggests that Helicobacter organisms are linked to IBD as well as to gastric and colon cancer. Therefore, the focus of this study was to evaluate the effect of lipopolysaccharide (LPS) isolated from Helicobacter on modulating the DNA repair system. We used an in vitro model represented by two colon carcinoma cell lines, the DNA repair-proficient SW480 and the DNA repair-deficient LoVo, and transfected with a UVC-irradiated psV-beta-galactosidase plasmid. We observed that LPS, by upregulating the expression of inducible nitric oxide (NO), leads to an increased NO release, demonstrating that LPS is able to interfere with the DNA repair machinery of intestinal cells, thus increasing the risk of permanent genotoxic effects. Springer-Verlag, 2010 LPS nationallicence DNA nationallicence Repair nationallicence Helicobacter nationallicence Intestinal cells nationallicence Cavallo Pasqua Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut Cianciulli Antonia Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut Mitolo Vincenzo Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut Panaro Maria Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut Clinical and Experimental Medicine Springer Milan 11/3(2011-09-01), 171-179 1591-8890 11:3<171 2011 11 10238 https://doi.org/10.1007/s10238-010-0118-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-010-0118-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cavallo Pasqua Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut NATIONALLICENCE 700 1- Cianciulli Antonia Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut NATIONALLICENCE 700 1- Mitolo Vincenzo Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut NATIONALLICENCE 700 1- Panaro Maria Department of Human Anatomy and Histology, University of Bari, P.zza G. Cesare, 11, 70124, Bari, Italy aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/3(2011-09-01), 171-179 1591-8890 11:3<171 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer