caa a22 4500 44535481X CHVBK 20180317142856.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10238-011-0134-9 doi (NATIONALLICENCE)springer-10.1007/s10238-011-0134-9 Expressions of IL-22 in circulating CD4+/CD8+ T cells and their correlation with disease activity in SLE patients [Elektronische Daten] [Wei-Zi Qin, Li-Li Chen, Hai-Feng Pan, Rui-Xue Leng, Zhi-Min Zhai, Chao Wang, Ruo-Jie Li, Song Wang, Hui-Ping Wang, Dong-Qing Ye] Recently, Th17 cell-associated responses have received growing attention; however, the role of IL-22 (a cytokines also produced by Th17 cells) in the pathogenesis of systemic lupus erythematosus (SLE) has not been widely explored. In this study, we analyze the frequencies of IL-22-positive CD4+/CD8+ T cells in peripheral blood mononuclear cells (PBMCs) from patients with SLE and their correlations with disease activity and clinical data. Five-color flow cytometry (FCM) was used to assess IL-22 production of CD4+/CD8+ T cells in PBMCs from 31 patients with SLE and 22 healthy control subjects, following stimulation ex vivo with phorbol 12-myristate 13-acetate and ionomycin for 4h. Results showed that the percentages of IL-22-positive CD4+ T cells were increased in the PBMCs of patients with SLE compared with healthy control subjects, whereas there were no significant differences in the percentages of IL-22-positive CD8+ T cells. There was a strong positive correlation between the proportion of CD4+ T cells expressing IL-22 and SLEDAI score (r s=0.65, P<0.001). Furthermore, the frequencies of IL-22-positive CD4+ T cells were significantly higher in patients with SLE with nephritis than those without nephritis (Z=−2.72, P<0.01). In conclusion, increased frequencies of IL-22-positive CD4+ T cells in patients with SLE and positive correlation with SLEDAI score and lupus nephritis suggest that this cytokine may be implicated in the pathogenesis of the disease. Springer-Verlag, 2011 IL-22 nationallicence Peripheral blood mononuclear cells nationallicence Systemic lupus erythematosus nationallicence Qin Wei-Zi Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Chen Li-Li Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Pan Hai-Feng Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Leng Rui-Xue Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Zhai Zhi-Min The Second Affiliated Hospital of Anhui Medical University, 678 Furong Road, 230601, Hefei, Anhui, People's Republic of China aut Wang Chao Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Li Ruo-Jie Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Wang Song Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Wang Hui-Ping The Second Affiliated Hospital of Anhui Medical University, 678 Furong Road, 230601, Hefei, Anhui, People's Republic of China aut Ye Dong-Qing Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut Clinical and Experimental Medicine Springer Milan 11/4(2011-12-01), 245-250 1591-8890 11:4<245 2011 11 10238 https://doi.org/10.1007/s10238-011-0134-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-011-0134-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qin Wei-Zi Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Chen Li-Li Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Pan Hai-Feng Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Leng Rui-Xue Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Zhai Zhi-Min The Second Affiliated Hospital of Anhui Medical University, 678 Furong Road, 230601, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wang Chao Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Li Ruo-Jie Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wang Song Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wang Hui-Ping The Second Affiliated Hospital of Anhui Medical University, 678 Furong Road, 230601, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Ye Dong-Qing Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/4(2011-12-01), 245-250 1591-8890 11:4<245 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer