caa a22 4500 445354844 CHVBK 20180317142856.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10238-010-0130-5 doi (NATIONALLICENCE)springer-10.1007/s10238-010-0130-5 Analysis of differentially expressed genes in human rectal carcinoma using suppression subtractive hybridization [Elektronische Daten] [So-young Choi, Jun Jang, Kyung Kim] The existence and treatment of rectal cancer are important for the function of defecation and the quality of life. However, the precise mechanisms of rectal carcinogenesis remain unclear. To screen the overexpressed gene in rectal carcinoma, we performed suppressive subtractive hybridization (SSH) on rectal carcinoma cells and the corresponding normal rectal cells. A total of 64 recombinant clones were subjected to DNA sequencing analysis, and 9 known genes were found to overexpressed in the tumors compared with those of the normal tissues. The genes are ST3 beta-galactoside alpha-2,3-sialyltransferase (ST3GAL5), interferon-induced transmembrane protein 3 (IFITM3), platelet-derived growth factor A-associated protein 1 (PDAP1), AlkB alkylating repair homolog 3 (ALKBH3), nucleoside diphosphate linked moiety X (Nudix)-type motif 14 (NUDT14), calponin 2 (CNN2), mitogen-activated protein kinase 14 (MAPK14), aconitase 1 (ACO1), and selenophosphate synthetase 1 (SEPHS1). The expression profiles of the genes were further confirmed in rectal carcinoma cells and the corresponding normal rectal cells of 12 patients by quantitative real-time RT-PCR. Our results revealed that ST3GAL5, IFITM3, PDAP1, ALKBH3, NUDT14, CNN2, MAPK14, ACO1, and SEPHS1 may be involved in rectal carcinogenesis. Springer-Verlag, 2011 Rectal cancer nationallicence Gene expression nationallicence Suppression subtractive hybridization (SSH) nationallicence Choi So-young Department of Surgery, Sanbon Jeil Hospital, Sanbon-dong 1095-1, 435-040, Gunpo, Kyungki-do, Korea aut Jang Jun Department of Biochemistry, Inha University College of Medicine, Incheon, Korea aut Kim Kyung Department of Surgery, Inha University College of Medicine, Incheon, Korea aut Clinical and Experimental Medicine Springer Milan 11/4(2011-12-01), 219-226 1591-8890 11:4<219 2011 11 10238 https://doi.org/10.1007/s10238-010-0130-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-010-0130-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi So-young Department of Surgery, Sanbon Jeil Hospital, Sanbon-dong 1095-1, 435-040, Gunpo, Kyungki-do, Korea aut NATIONALLICENCE 700 1- Jang Jun Department of Biochemistry, Inha University College of Medicine, Incheon, Korea aut NATIONALLICENCE 700 1- Kim Kyung Department of Surgery, Inha University College of Medicine, Incheon, Korea aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/4(2011-12-01), 219-226 1591-8890 11:4<219 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer