caa a22 4500 445354887 CHVBK 20180317142856.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10238-010-0107-4 doi (NATIONALLICENCE)springer-10.1007/s10238-010-0107-4 DNA amplification on chromosome 13q31.1 correlated with poor prognosis in colorectal cancer [Elektronische Daten] [Tawiwan Sareeboot, Phaibul Punyarit, Songsak Petmitr] Colorectal cancer is a leading cause of cancer deaths worldwide. Genetic markers involved in prognosis of colorectal cancer are still being elucidated. In this study, genetic alterations associated with prognosis of colorectal cancer were determined using arbitrarily primed polymerase chain reaction (AP-PCR) and analyzed quantitatively by real-time PCR. Seven different DNA sequences, mapped on chromosomes 13q31.1, 9q31.1, 1q24, 4q31.3, 10q21, 11q13.4, and 13q13.3, were identified. Among these sequences, seven cases (23%) harbored DNA amplification in chromosome 13q31.1, and 9 (29%) and 7 (23%) presented genetic alterations in chromosome 1q24 and 11q13.4, respectively. Multivariate analysis showed that only DNA amplification in chromosome 13q31.1 was associated with poor survival among patients with colorectal cancer, with median survival time for chromosome 13q31.1 amplification versus no amplification of 64 versus 268weeks (P=0.001). This genetic alteration may have a prognostic role in colorectal cancer. Springer-Verlag, 2010 Colorectal cancer nationallicence 13q31.1 nationallicence Prognostic value nationallicence Sareeboot Tawiwan Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, 10400, Bangkok, Thailand aut Punyarit Phaibul Institute of Pathology, Phramongkutklao Medical Center, Bangkok, Thailand aut Petmitr Songsak Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, 10400, Bangkok, Thailand aut Clinical and Experimental Medicine Springer Milan 11/2(2011-06-01), 97-103 1591-8890 11:2<97 2011 11 10238 https://doi.org/10.1007/s10238-010-0107-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-010-0107-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sareeboot Tawiwan Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Punyarit Phaibul Institute of Pathology, Phramongkutklao Medical Center, Bangkok, Thailand aut NATIONALLICENCE 700 1- Petmitr Songsak Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, 10400, Bangkok, Thailand aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/2(2011-06-01), 97-103 1591-8890 11:2<97 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer