caa a22 4500 445354895 CHVBK 20180317142856.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10238-010-0110-9 doi (NATIONALLICENCE)springer-10.1007/s10238-010-0110-9 Wnt/beta-Catenin pathway in human glioma: expression pattern and clinical/prognostic correlations [Elektronische Daten] [Ce Liu, Yanyang Tu, Xiaoyang Sun, Jian Jiang, Xiaodong Jin, Xiangfei Bo, Zhengming Li, Aimiao Bian, Xiaodong Wang, Dai Liu, Zhengmei Wang, Lianshu Ding] Gliomas are the most common primary intracranial tumors. Understanding the molecular basis of gliomas' progression is required to develop more effective therapies. The Wnt/β-catenin signaling cascade is an important signal transduction pathway in human cancers. Although, overactivation of this pathway is a hallmark of several forms of cancer, little is known about its role in human gliomas. Here, we aimed to determine the clinical significance of Wnt/β-catenin pathway components in gliomas. Immunohistochemical staining was performed to detect the expression patterns of Wnt1, β-catenin and Cyclin D1 in the biopsies from 96 patients with primary gliomas. Kaplan-Meier survival and Cox regression analyses were performed to evaluate the prognosis of patients. Cytoplasmic staining pattern of Wnt1, membranous, cytoplasmic and nuclear accumulation of β-catenin, and nuclear localization of Cyclin D1 were demonstrated by immunohistochemical staining. The Wnt1 expression significantly correlated with the expression of Cyclin D1 (P<0.0001). The ratio of tumors with a cytoplasmic-nuclear pattern or a cytoplasmic pattern of β-catenin was significantly higher in Wnt1-positive (P<0.01) and Cyclin D1-positive (P<0.01) tumors than in Wnt1-negative and Cyclin D1-negative tumors, respectively. The protein expression levels of Wnt1, β-catenin and Cyclin D1 were all positively correlated with the Karnofsky performance scale (KPS) score and World Health Organization (WHO) grades of patients with gliomas. Furthermore, Wnt1, cytoplasmic-nuclear β-catenin and Cyclin D1 status were all the independent prognostic factors for glioma patients (P=0.01, 0.007 and 0.005, respectively). These results provide convincing evidence that the Wnt/β-catenin pathway correlated closely with the progression of gliomas and might be a novel prognostic marker for this neoplasm. Springer-Verlag, 2010 Glioma nationallicence Wnt/beta-Catenin nationallicence Cyclin D1 nationallicence Immunohistochemical staining nationallicence Prognosis nationallicence Liu Ce Department of Neurosurgery, The 309th Hospital of PLA, 100091, Beijing, People's Republic of China aut Tu Yanyang Department of Emergency, Tangdu Hospital, The Fourth Military Medical University, 710038, Xi'an, People's Republic of China aut Sun Xiaoyang Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Jiang Jian Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Jin Xiaodong Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Bo Xiangfei Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Li Zhengming Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Bian Aimiao Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Wang Xiaodong Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Liu Dai Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Wang Zhengmei Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Ding Lianshu Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut Clinical and Experimental Medicine Springer Milan 11/2(2011-06-01), 105-112 1591-8890 11:2<105 2011 11 10238 https://doi.org/10.1007/s10238-010-0110-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-010-0110-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Ce Department of Neurosurgery, The 309th Hospital of PLA, 100091, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Tu Yanyang Department of Emergency, Tangdu Hospital, The Fourth Military Medical University, 710038, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Sun Xiaoyang Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Jian Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Jin Xiaodong Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Bo Xiangfei Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Li Zhengming Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Bian Aimiao Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiaodong Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Liu Dai Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhengmei Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 700 1- Ding Lianshu Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, 223300, Huaian City, People's Republic of China aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/2(2011-06-01), 105-112 1591-8890 11:2<105 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer