caa a22 4500 445354925 CHVBK 20180317142856.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10238-010-0113-6 doi (NATIONALLICENCE)springer-10.1007/s10238-010-0113-6 Glycosylation of mucins present in gastric juice: the effect of helicobacter pylori eradication treatment [Elektronische Daten] [Iwona Radziejewska, Małgorzata Borzym-Kluczyk, Zbigniew Namiot, Ewa Stefańska] It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of eradication therapy. Gastric juices were taken from 13 clinical patients and subjected to analysis. Pooled fractions of the void volume obtained after gel filtration were subjected to ELISA tests. To assess the relative amounts of carbohydrate structures, lectins and monoclonal antibodies were used. Changes in the level of MUC 1 and MUC 5AC mucins and of carbohydrate structures, which are suggested to be receptors for Helicobacter pylori adhesins, were observed by the end of the eradication treatment. Our results support the idea about the involvement of MUC 5AC and MUC 1 with some specific sugar structures in the mechanism of Helicobacter pylori infection. The Author(s), 2010 Gastric juices nationallicence Glycosylation nationallicence Lectins nationallicence MUC 1 mucin nationallicence MUC 5AC mucin nationallicence Radziejewska Iwona Department of Medical Chemistry, Medical University of Białystok, ul. Mickiewicza 2a, 15-222, Białystok 8, Poland aut Borzym-Kluczyk Małgorzata Department of Pharmaceutical Biochemistry, Medical University of Białystok, Białystok, Poland aut Namiot Zbigniew Department of Medicine and Gastroenterology, Regional Hospital of Białystok, Białystok, Poland aut Stefańska Ewa Department of Dietetics and Nutrition, Medical University of Białystok, Białystok, Poland aut Clinical and Experimental Medicine Springer Milan 11/2(2011-06-01), 81-88 1591-8890 11:2<81 2011 11 10238 https://doi.org/10.1007/s10238-010-0113-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10238-010-0113-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Radziejewska Iwona Department of Medical Chemistry, Medical University of Białystok, ul. Mickiewicza 2a, 15-222, Białystok 8, Poland aut NATIONALLICENCE 700 1- Borzym-Kluczyk Małgorzata Department of Pharmaceutical Biochemistry, Medical University of Białystok, Białystok, Poland aut NATIONALLICENCE 700 1- Namiot Zbigniew Department of Medicine and Gastroenterology, Regional Hospital of Białystok, Białystok, Poland aut NATIONALLICENCE 700 1- Stefańska Ewa Department of Dietetics and Nutrition, Medical University of Białystok, Białystok, Poland aut NATIONALLICENCE 773 0- Clinical and Experimental Medicine Springer Milan 11/2(2011-06-01), 81-88 1591-8890 11:2<81 2011 11 10238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer