caa a22 4500 445355778 CHVBK 20180317142859.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10072-011-0711-4 doi (NATIONALLICENCE)springer-10.1007/s10072-011-0711-4 Effects of conjugated linoleic acid on cleavage of amyloid precursor protein via PPARγ [Elektronische Daten] [Yi-Chen Li, Qing Chen, Xian-Zi Wan, Xiang-Ling Yang, Xin Liu, Ling Zhong] Conjugated linoleic acid (CLA) plays important roles in physiological conditions. The aim of present study was to explore the effects of CLA on the cleavage of amyloid precursor protein (APP) and the potential mechanism involved. The effects of CLA on intracellular APP, BACE1 (β-site APP Cleaving Enzyme1, BACE1), a disintegrin and metalloprotease (ADAM10) and extracellular sAPPα (soluble) were analyzed by RT-PCR, Western blot and ELISA in SH-SY5Y cells. Our study indicated that CLA significantly decreased the expression of BACE1 and increased the extracellular secretion of sAPPα, but not affected the levels of APP and ADAM10. The study also revealed that the nuclear receptor peroxisome proliferators activated receptor γ (PPARγ) played an important role in the CLA-induced intracellular BACE1 decrease, as well as the extracellular sAPPα increase through knockdown of PPARγ transcription using siRNA. We hypothesize that CLA acts as an agonist or ligand, which binds with PPARγ and leads to the increase in APP cleavage via α-secretase-mediated pathway and the decrease in the deposition of Aβ. Springer-Verlag, 2011 CLA nationallicence APP nationallicence BACE1 nationallicence sAPPα nationallicence PPARγ nationallicence Li Yi-Chen School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut Chen Qing School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut Wan Xian-Zi School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut Yang Xiang-Ling School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut Liu Xin School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut Zhong Ling School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut Neurological Sciences Springer Milan 32/6(2011-12-01), 1095-1101 1590-1874 32:6<1095 2011 32 10072 https://doi.org/10.1007/s10072-011-0711-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10072-011-0711-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yi-Chen School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Chen Qing School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Wan Xian-Zi School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Yang Xiang-Ling School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Liu Xin School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Zhong Ling School of Pharmaceutical Sciences, Rm 211, Sun Yat-sen University, 132 Waihuan East Road, Higher Education Mega Center, 510006, Guangzhou, Guangdong, China aut NATIONALLICENCE 773 0- Neurological Sciences Springer Milan 32/6(2011-12-01), 1095-1101 1590-1874 32:6<1095 2011 32 10072 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer