caa a22 4500 445356065 CHVBK 20180317142900.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10072-011-0512-9 doi (NATIONALLICENCE)springer-10.1007/s10072-011-0512-9 Therapeutic time window for the neuroprotective effects of NGF when administered after focal cerebral ischemia [Elektronische Daten] [Ji-Ping Yang, Huai-Jun Liu, Hua Yang, Ping-Yong Feng] In the present study, we evaluated the neuroprotection time window for nerve growth factor (NGF) after ischemia/reperfusion brain injury in rabbits as related to this anti-apoptosis mechanism. Male New Zealand rabbits were subjected to 2h of middle cerebral artery occlusion (MCAO), followed by 70h of reperfusion. NGF was administered after injury to evaluate the time window. Neurological deficits, infarct volume, neural cell apoptosis and expressions of caspase-3 and Bcl-2 were measured. Compared to saline-treated control, NGF treatment at 2, 3 and 5h after MCAO significantly reduced infarct volume, neural cell apoptosis and expression of caspase-3 (P<0.01), up-regulated the expression of Bcl-2 and improved functional recovery (P<0.01). However, treatment at latter time points did not produce significant neuroprotection. Neuroprotection treatment with NGF provides an extended time window of up to 5 h after ischemia/reperfusion brain injury, in part by attenuating the apoptosis. Springer-Verlag, 2011 Focal cerebral ischemia nationallicence Nerve growth factor nationallicence Neuroprotection nationallicence Time window nationallicence Bcl-2 nationallicence Apoptosis nationallicence Yang Ji-Ping Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut Liu Huai-Jun Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut Yang Hua Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut Feng Ping-Yong Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut Neurological Sciences Springer Milan 32/3(2011-06-01), 433-441 1590-1874 32:3<433 2011 32 10072 https://doi.org/10.1007/s10072-011-0512-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10072-011-0512-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Ji-Ping Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut NATIONALLICENCE 700 1- Liu Huai-Jun Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut NATIONALLICENCE 700 1- Yang Hua Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut NATIONALLICENCE 700 1- Feng Ping-Yong Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, 050000, Shijiazhuang, Hebei Province, China aut NATIONALLICENCE 773 0- Neurological Sciences Springer Milan 32/3(2011-06-01), 433-441 1590-1874 32:3<433 2011 32 10072 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer