caa a22 4500 445357096 CHVBK 20180317142903.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10072-011-0608-2 doi (NATIONALLICENCE)springer-10.1007/s10072-011-0608-2 Bone marrow mesenchymal stem cells can be mobilized into peripheral blood by G-CSF in vivo and integrate into traumatically injured cerebral tissue [Elektronische Daten] [Jun Deng, Zhong-min Zou, Tao-li Zhou, Yong-ping Su, Guo-ping Ai, Jun-ping Wang, Hui Xu, Shi-wu Dong] The efficacy of granulocyte colony-stimulating factor (G-CSF) in mobilizing mesenchymal stem cells (MSCs) into peripheral blood (PB) and the ability of PB-MSCs incorporated into injured brain were tested. Colony forming, cell phenotype and differentiation potential of mouse MSCs mobilized by G-CSF (40μg/kg) were evaluated. Mortality and pathological changes in mice with serious craniocerebral trauma plus G-CSF treatment (40μg/kg) were investigated. Bone marrow (BM) cells derived from GFP mice were fractionated into MSCs, hematopoietic stem cells (HSCs), and non-MSC/HSCs using magnetic beads and adherent culture. The resultant cell populations were transplanted into injured mice. The in vivo integration and differentiation of the transplanted cells were detected immunocytochemically. The expression of SDF-1 in injured area of brain was tested by Western blot. G-CSF was able to mobilize MSCs into PB (fourfold increase). PB-MSCs possessed similar characteristics as BM-MSCs in terms of colony formation, the expression pattern of CD73, 44, 90, 106, 31 and 45, and multipotential of differentiation. Accumulative total mice mortality was lower in TG group (5/14) than that in T group (7/14). It was MSCs, not HSCs or non-MSC/HSC cells integrated into the damaged cerebral tissue and differentiated into cells expressing neural markers. Increased SDF-1 expression in injured area of brain was confirmed, which could facilitate the homing of MSCs to brain. G-CSF can mobilize MSCs into PB and MSCs in PB can integrate into injured cerebral tissue and transdifferentiated into neural cells and may benefit the repair of trauma. Springer-Verlag, 2011 Mesenchymal stem cells nationallicence Granulocyte colony-stimulating factor nationallicence Stem cell mobilization nationallicence Cell differentiation nationallicence Deng Jun Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut Zou Zhong-min Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut Zhou Tao-li Department of Pathogenic Biology, School of Medical Science, The Third Military Medical University, 400038, Chongqing, China aut Su Yong-ping Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut Ai Guo-ping Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut Wang Jun-ping Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut Xu Hui Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut Dong Shi-wu Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut Neurological Sciences Springer Milan 32/4(2011-08-01), 641-651 1590-1874 32:4<641 2011 32 10072 https://doi.org/10.1007/s10072-011-0608-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10072-011-0608-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deng Jun Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Zou Zhong-min Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Zhou Tao-li Department of Pathogenic Biology, School of Medical Science, The Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Su Yong-ping Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Ai Guo-ping Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Wang Jun-ping Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Xu Hui Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Dong Shi-wu Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, 400038, Chongqing, China aut NATIONALLICENCE 773 0- Neurological Sciences Springer Milan 32/4(2011-08-01), 641-651 1590-1874 32:4<641 2011 32 10072 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer