caa a22 4500 445357339 CHVBK 20180317142904.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10072-011-0485-8 doi (NATIONALLICENCE)springer-10.1007/s10072-011-0485-8 In vitro effects of Cyberknife-driven intermittent irradiation on glioblastoma cell lines [Elektronische Daten] [Alessandra Canazza, Ugo De Grazia, Luisa Fumagalli, Lorenzo Brait, Francesco Ghielmetti, Laura Fariselli, Danilo Croci, Andrea Salmaggi, Emilio Ciusani] Radiosurgery is used increasingly upon recurrence of high-grade gliomas to deliver a high dose of focused radiation to a defined target. The purpose of our study was to compare intermittent irradiation (IIR) by using a CyberKnife (CK) with continuous irradiation (CIR) by using a conventional linear accelerator (LINAC). A significant decrease in surviving fraction was observed after IIR irradiation compared with after CIR at a dose of 8Gy. Three hours after irradiation, most of the DNA damage was repaired in U87. Slightly higher basal levels of Ku70/80 mRNA were found in U87 compared with A172, while radiation treatment induced only minor regulation of Ku70/80 and Rad51 transcription in either cell lines. IIR treatment using CK significantly decreased the survival in U87 and A172 compared with CIR. Although the two cell lines differed in DNA repair capability, the role of Ku70/80 and Rad51 in the cell line radiosensitivity seemed marginal. Springer-Verlag, 2011 Glioma nationallicence Radiation nationallicence DNA-damage nationallicence Repair nationallicence Comet assay nationallicence CyberKnife nationallicence Canazza Alessandra Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut De Grazia Ugo Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut Fumagalli Luisa Radiotherapy Unit, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut Brait Lorenzo Cyberknife Unit, Centro Diagnostico Italiano, Via Saint Bon 20, 20147, Milan, Italy aut Ghielmetti Francesco Radiotherapy Unit, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut Fariselli Laura Radiotherapy Unit, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut Croci Danilo Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut Salmaggi Andrea Department of Neurooncology, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut Ciusani Emilio Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut Neurological Sciences Springer Milan 32/4(2011-08-01), 579-588 1590-1874 32:4<579 2011 32 10072 https://doi.org/10.1007/s10072-011-0485-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10072-011-0485-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Canazza Alessandra Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 700 1- De Grazia Ugo Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Fumagalli Luisa Radiotherapy Unit, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Brait Lorenzo Cyberknife Unit, Centro Diagnostico Italiano, Via Saint Bon 20, 20147, Milan, Italy aut NATIONALLICENCE 700 1- Ghielmetti Francesco Radiotherapy Unit, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Fariselli Laura Radiotherapy Unit, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Croci Danilo Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Salmaggi Andrea Department of Neurooncology, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Ciusani Emilio Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico "C. Besta”, Via Celoria 11, 20133, Milan, Italy aut NATIONALLICENCE 773 0- Neurological Sciences Springer Milan 32/4(2011-08-01), 579-588 1590-1874 32:4<579 2011 32 10072 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer