caa a22 4500 445358025 CHVBK 20180317142906.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10072-010-0359-5 doi (NATIONALLICENCE)springer-10.1007/s10072-010-0359-5 Ischemic preconditioning attenuates of ischemia-induced degradation of spectrin and tau: implications for ischemic tolerance [Elektronische Daten] [Takayuki Nakajima, Syoichi Ochi, Chika Oda, Maki Ishii, Kazushige Ogawa] Global ischemia selectively induces CA1 neuronal death in the hippocampus. Pretreatment with non-lethal ischemia (i.e. ischemic preconditioning) prevents CA1 neuronal death induced by lethal ischemia. While ischemic tolerance is a well-known phenomenon, the underlying molecular mechanisms are not fully understood. Cytoskeletal proteins including α-spectrin, tau, and microtubule-associated protein 2 (MAP-2) are indispensable for the maintenance of neuronal homeostasis. Here, we report the effects of ischemic preconditioning on the ischemia-induced degradation of cytoskeletal proteins α-spectrin, tau, and MAP-2 in the rat CA1 region. We found that most neurons of the CA1 region had died after 5min of ischemia. However, exposing the brain to 3min of ischemic preconditioning 3days earlier significantly reduced the number of neuronal death. A significant degradation of α-spectrin and tau, but not of MAP-2, was found in the CA1 region after 5min of ischemia. Ischemic preconditioning attenuated the ischemia-induced massive degradation of α-spectrin and tau. Our results suggest that the attenuation of ischemia-induced degradation of α-spectrin and tau by ischemic preconditioning may be associated with the neuroprotective mechanism of the ischemic tolerance. Springer-Verlag, 2010 Ischemic tolerance nationallicence Spectrin nationallicence Tau nationallicence MAP-2 nationallicence Hippocampus nationallicence Nakajima Takayuki Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut Ochi Syoichi Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut Oda Chika Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut Ishii Maki Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut Ogawa Kazushige Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut Neurological Sciences Springer Milan 32/2(2011-04-01), 229-239 1590-1874 32:2<229 2011 32 10072 https://doi.org/10.1007/s10072-010-0359-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10072-010-0359-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakajima Takayuki Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut NATIONALLICENCE 700 1- Ochi Syoichi Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut NATIONALLICENCE 700 1- Oda Chika Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut NATIONALLICENCE 700 1- Ishii Maki Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut NATIONALLICENCE 700 1- Ogawa Kazushige Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ohraikita, 598-8531, Izumisano, Osaka, Japan aut NATIONALLICENCE 773 0- Neurological Sciences Springer Milan 32/2(2011-04-01), 229-239 1590-1874 32:2<229 2011 32 10072 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer