caa a22 4500 445358092 CHVBK 20180317142906.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10072-010-0340-3 doi (NATIONALLICENCE)springer-10.1007/s10072-010-0340-3 Proteomics analysis of MPP+-induced apoptosis in SH-SY5Y cells [Elektronische Daten] [Hongrong Xie, Ming Chang, Xinyu Hu, Danping Wang, Mingxiu Tian, Guoyi Li, Huiyi Jiang, Ying Wang, Zhong Dong, Yuhua Zhang, Linsen Hu] Accumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (MPP+) to induce apoptotic neuronal injury. 2D-DIGE followed by MALDI-TOF-MS was used to determine the changing protein levels. Proteomics analysis revealed that 22 proteins were differentially altered in MPP+-treated SH-SY5Y cells, of which 7 were up-regulated proteins and 15 were down-regulated proteins, respectively. Three protein spots were unambiguously identified as sorcin, annexin V, and ribosomal protein P0. The three proteins showed a significant increase in level, suggesting a role in MPP+-induced apoptosis. The functional roles of these three proteins collectively indicate that multiple mechanisms are pertinent in the underlying pathogenesis of Parkinson's disease (PD), such as apoptosis, calcium homeostasis, and DNA insults. Springer-Verlag, 2010 Proteomics nationallicence SH-SY5Y cells nationallicence MPP+ nationallicence Sorcin nationallicence Annexin V nationallicence Ribosomal protein P0 nationallicence Xie Hongrong Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Chang Ming Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Hu Xinyu The Second Hospital, Jilin University, 130041, Changchun, China aut Wang Danping Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Tian Mingxiu Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Li Guoyi Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Jiang Huiyi Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Wang Ying Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Dong Zhong Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Zhang Yuhua Department of Computerized Tomography, Daqing Oilfield General Hospital, 163001, Daqing, China aut Hu Linsen Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut Neurological Sciences Springer Milan 32/2(2011-04-01), 221-228 1590-1874 32:2<221 2011 32 10072 https://doi.org/10.1007/s10072-010-0340-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10072-010-0340-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xie Hongrong Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Chang Ming Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Hu Xinyu The Second Hospital, Jilin University, 130041, Changchun, China aut NATIONALLICENCE 700 1- Wang Danping Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Tian Mingxiu Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Li Guoyi Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Huiyi Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang Ying Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Dong Zhong Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yuhua Department of Computerized Tomography, Daqing Oilfield General Hospital, 163001, Daqing, China aut NATIONALLICENCE 700 1- Hu Linsen Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Neurological Sciences Springer Milan 32/2(2011-04-01), 221-228 1590-1874 32:2<221 2011 32 10072 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer