caa a22 4500 445358246 CHVBK 20180317142907.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10072-010-0394-2 doi (NATIONALLICENCE)springer-10.1007/s10072-010-0394-2 Low doses of 3-nitropropionic acid in vivo induce damage in mouse skeletal muscle [Elektronische Daten] [Elizabeth Hernández-Echeagaray, Nancy González, Angélica Ruelas, Ernesto Mendoza, Erika Rodríguez-Martínez, Rafael Antuna-Bizarro] Mitochondrial alterations are believed to play a critical role in the pathophysiology of neurodegenerative diseases and in some well-described myopathies. In the present study, we evaluated muscle changes in vivo after blocking the mitochondrial complex II of the respiratory chain by using 3-nitropropionic acid (3-NP). This neurotoxin has been used as a pharmacological tool in animal models to address some of the metabolic modifications that might underlie central neurodegeneration; however, changes in peripheral musculature have not been documented. We believe that skeletal muscles must be affected because their integrity highly depends on oxidative metabolism. Therefore, histochemical, ultrastructural, and biochemical changes were studied in the muscles of mice treated with low doses of 3-NP (15mg/kg, i.p., for 5days). 3-NP-treated mice displayed changes in alkaline phosphatase (APase), succinic dehydrogenase (SDH), and cytochrome c oxidase (COX) levels in the gracilis and gastrocnemius muscles. These changes were statistically significant for APase and SDH in both muscles and for COX only in the gastrocnemius. No significant alterations in acetylcholinesterase (AChE) expression were observed in either muscle. Analysis of the muscle ultrastructure revealed mitochondrial atrophy as well as sarcomere and nuclei disorganization. At the biochemical level, nitric oxide (NO) and lipid peroxidation (LPO) changed in the muscles of 3-NP-treated mice, suggesting metabolic alterations due to oxidative stress. Early damage in the striatal tissue and behavioral modifications are also documented. Springer-Verlag, 2010 3-NP nationallicence Muscle nationallicence SDH nationallicence Neurodegeneration nationallicence Oxidative phosphorylation nationallicence Hernández-Echeagaray Elizabeth Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut González Nancy Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut Ruelas Angélica Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut Mendoza Ernesto Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut Rodríguez-Martínez Erika Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut Antuna-Bizarro Rafael Instituto Nacional de Pediatría, C. P. 04530, Mexico city, Mexico aut Neurological Sciences Springer Milan 32/2(2011-04-01), 241-254 1590-1874 32:2<241 2011 32 10072 https://doi.org/10.1007/s10072-010-0394-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10072-010-0394-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hernández-Echeagaray Elizabeth Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut NATIONALLICENCE 700 1- González Nancy Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut NATIONALLICENCE 700 1- Ruelas Angélica Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut NATIONALLICENCE 700 1- Mendoza Ernesto Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut NATIONALLICENCE 700 1- Rodríguez-Martínez Erika Laboratorio de Neurofisiología del Desarrollo y la Neurodegeneración, Unidad de Biomedicina, FES-I, Universidad Nacional Autónoma de México, Av. De Los Barrios # 1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Mexico aut NATIONALLICENCE 700 1- Antuna-Bizarro Rafael Instituto Nacional de Pediatría, C. P. 04530, Mexico city, Mexico aut NATIONALLICENCE 773 0- Neurological Sciences Springer Milan 32/2(2011-04-01), 241-254 1590-1874 32:2<241 2011 32 10072 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer