caa a22 4500 445363894 CHVBK 20180317142923.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10397-011-0658-8 doi (NATIONALLICENCE)springer-10.1007/s10397-011-0658-8 Intraperitoneal injection of cultured mesothelial cells decrease CO2 pneumoperitoneum-enhanced adhesions in a laparoscopic mouse model [Elektronische Daten] [J. Verguts, A. Coosemans, R. Corona, M. Praet, K. Mailova, P. Koninckx] Mesothelial cells are involved in peritoneal repair, and they modulate adhesion formation. Intraperitoneal injection of cultured mesothelial cells can decrease adhesion formation. The importance of the amount of cells, of the culture methods or of the origin of the cells is still unknown. Female Balb/c mice were used in a CO2 pneumoperitoneum-enhanced adhesion formation model. Mesothelial cells were obtained from Balb/c mice and cultured over a 3-week period. Following a pilot experiment (n = 10) with injection of 400,000 mesothelial cells a dose-response study was performed with 400,000; 133,000; and 44,000 cells (n = 40). Adhesion formation decreased after the injection of cultured mesothelial cells as evidenced in the first (proportion p < 0.046, total adhesions: NS) and second experiment (p < 0.001 for proportion and total adhesions). The reduction was moreover dose dependent with a half maximal effect with some 100,000 cells. Intraperitoneal injection of cultured mesothelial cells can decrease CO2 pneumoperitoneum-enhanced adhesion formation in mice and the effect is dose dependent, the half maximum effect being obtained with some 100,000 cells, a number which is surprisingly similar to the number of cells harvested from one mouse. Springer-Verlag, 2011 Laparoscopy nationallicence Adhesions nationallicence Mesothelial cells nationallicence Mouse model nationallicence Verguts J. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut Coosemans A. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut Corona R. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut Praet M. Department of Pathology, UZ Gent, Gent, Belgium aut Mailova K. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut Koninckx P. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut Gynecological Surgery Springer-Verlag 8/4(2011-11-01), 409-414 1613-2076 8:4<409 2011 8 10397 https://doi.org/10.1007/s10397-011-0658-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10397-011-0658-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verguts J. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut NATIONALLICENCE 700 1- Coosemans A. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut NATIONALLICENCE 700 1- Corona R. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut NATIONALLICENCE 700 1- Praet M. Department of Pathology, UZ Gent, Gent, Belgium aut NATIONALLICENCE 700 1- Mailova K. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut NATIONALLICENCE 700 1- Koninckx P. Department of Obstetrics and Gynecology, UZ Leuven, Campus Gasthuisberg, Leuven, Belgium aut NATIONALLICENCE 773 0- Gynecological Surgery Springer-Verlag 8/4(2011-11-01), 409-414 1613-2076 8:4<409 2011 8 10397 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer