caa a22 4500 445364521 CHVBK 20180317142925.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10048-011-0290-9 doi (NATIONALLICENCE)springer-10.1007/s10048-011-0290-9 Differentially expressed genes in hypothalamus in relation to genomic regions under selection in two chicken lines resulting from divergent selection for high or low body weight [Elektronische Daten] [Sojeong Ka, Frank Albert, D. Denbow, Svante Pääbo, Paul Siegel, Leif Andersson, Finn Hallböök] Long-term divergent selection for low or high body weight from the same founder population has generated two extremely divergent lines of chickens, the high- (HWS) and low-weight (LWS) selected lines. At selection age (56days), the lines differ by more than nine times in body weight. The HWS line chickens are compulsive feeders, whereas in the LWS line, some individuals are anorexic and others have very low appetite. Previous studies have implicated the central nervous system and particularly the hypothalamus in these behavioural differences. Here, we compared the mRNA expression in hypothalamus tissue from chickens on day4 post-hatch using oligonucleotide arrays and found that the divergent selection had resulted in minor but multiple expression differences. Differentially expressed genes were enriched in processes ‘DNA metabolism, repair, induction of apoptosis and metabolism'. Several differentially expressed genes participate in the regulation of neuronal plasticity and development, including apoptosis, or are neurotransmittor receptor subtypes. Less change was seen when comparing hypothalamic neuropeptide mediators of appetite such as the melanocortin receptors. The genomic locations of these differentially expressed genes were then compared to the locations of growth QTLs and to a genome-wide map of chromosomal regions that have been under divergent selection between the lines. The results indicate which differentially expressed hypothalamic genes have responded to the divergent selection and that the results predict that it is more likely to find causative genes among these most differentially expressed genes. Because of such differential gene expression in hypothalamus, the lines may adapt behaviourally different particularly to the post-hatch situation when independent feeding to obtain energy is established. Springer-Verlag, 2011 Anorexia nationallicence Body weight nationallicence Chicken nationallicence Divergent selection nationallicence Feeding behaviour nationallicence Gene expression nationallicence Hypothalamus nationallicence Ka Sojeong Department of Neuroscience, Uppsala University, Uppsala, Sweden aut Albert Frank Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany aut Denbow D. Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA aut Pääbo Svante Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany aut Siegel Paul Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA aut Andersson Leif Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden aut Hallböök Finn Department of Neuroscience, Uppsala University, Uppsala, Sweden aut neurogenetics Springer-Verlag 12/3(2011-08-01), 211-221 1364-6745 12:3<211 2011 12 10048 https://doi.org/10.1007/s10048-011-0290-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10048-011-0290-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ka Sojeong Department of Neuroscience, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Albert Frank Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany aut NATIONALLICENCE 700 1- Denbow D. Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Pääbo Svante Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany aut NATIONALLICENCE 700 1- Siegel Paul Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Andersson Leif Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Hallböök Finn Department of Neuroscience, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 773 0- neurogenetics Springer-Verlag 12/3(2011-08-01), 211-221 1364-6745 12:3<211 2011 12 10048 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer