caa a22 4500 445372796 CHVBK 20180317142952.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00540-010-1037-8 doi (NATIONALLICENCE)springer-10.1007/s00540-010-1037-8 Total parenteral-nutrition-mediated dendritic-cell activation and infiltration into the small intestine in a rat model [Elektronische Daten] [Satoshi Hagiwara, Hideo Iwasaka, Junya Kusaka, Nobuhiko Asai, Tomohisa Uchida, Takayuki Noguchi] Purpose: Total parenteral nutrition (TPN) is commonly carried out in the clinical setting. However, effects of TPN on the immune system, including dendritic cells (DC), are not well understood. The purpose of this study was to determine whether TPN affects DC activation and infiltration into the intestinal barrier. Methods: Male Wistar rats were given conventional nutrition (CN) or TPN for 7days. DCs were visualized by immunohistochemistry. Levels of nucleotide-binding oligomerization domain protein 2 (NOD2) and high-mobility group box 1 (HMGB1) protein were assessed by Western blot. Results: The number of DCs at the small intestinal barrier was significantly increased in the TPN group (9.2±3.1 cells/microscopic field) compared with the CN group (0.5±0.6 cells/microscopic field; p<0.05), as were protein expression levels of NOD2 and HMGB1. Conclusion: These results suggest that TPN increases activation and infiltration of DCs into the small intestine, potentially involving an increase in NOD2 and HMGB1 levels in the small intestine. Japanese Society of Anesthesiologists, 2010 HMGB1 nationallicence NOD2 nationallicence Nutrition nationallicence Intestinal barrier nationallicence Immunity nationallicence Hagiwara Satoshi Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Iwasaka Hideo Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Kusaka Junya Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Asai Nobuhiko Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Uchida Tomohisa Department of Molecular Pathology, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Noguchi Takayuki Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Journal of Anesthesia Springer Japan 25/1(2011-02-01), 57-64 0913-8668 25:1<57 2011 25 540 https://doi.org/10.1007/s00540-010-1037-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00540-010-1037-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hagiwara Satoshi Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Iwasaka Hideo Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Kusaka Junya Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Asai Nobuhiko Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Uchida Tomohisa Department of Molecular Pathology, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Noguchi Takayuki Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 773 0- Journal of Anesthesia Springer Japan 25/1(2011-02-01), 57-64 0913-8668 25:1<57 2011 25 540 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer