caa a22 4500 44537294X CHVBK 20180317142953.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00540-010-1071-6 doi (NATIONALLICENCE)springer-10.1007/s00540-010-1071-6 Effects of orexin-A on propofol anesthesia in rats [Elektronische Daten] [Tetsuro Shirasaka, Tetsu Yonaha, Shin Onizuka, Isao Tsuneyoshi] Purpose: An active sleep homeostatic process is present during propofol anesthesia. Activation of the orexin system induces wakefulness, and inhibition of the orexin system causes narcolepsy. We hypothesized that orexin would affect propofol anesthesia. Methods: The effects of an intracerebroventricular (i.c.v.) injection of orexin-A (OXA) or an orexin-1 (OX-1) receptor antagonist, SB-334867, on the times to the loss and return of the righting reflex induced by propofol were examined in Wistar rats. The effects of propofol or OXA on norepinephrine (NE) and dopamine (DA) release from the prefrontal cortex (PFC) were examined using in vivo microdialysis. Results: An i.c.v. injection of OXA (1nmol) decreased the time to emergence from propofol anesthesia mediated by the OX-1 receptor without changing anesthetic induction (n=8). An i.c.v. injection of SB-334867 (5 and 50nmol) increased the time to emergence from propofol anesthesia without changing anesthetic induction (n=8). Intravenous infusion of propofol decreased NE (48±8%; n=8) and DA (61.2±11%; n=8) release from PFC mediated by the GABAA receptor. An i.c.v. injection of OXA reversed the decreases in NE and DA release induced by propofol mediated by the OX-1 receptor (n=8). Conclusion: These results indicate that the orexin system may accelerate the emergence from propofol anesthesia associated with increases in the central noradrenergic and dopaminergic activity. Japanese Society of Anesthesiologists, 2010 Orexin nationallicence Propofol nationallicence Norepinephrine nationallicence Dopamine nationallicence Microdialysis nationallicence Shirasaka Tetsuro Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut Yonaha Tetsu Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut Onizuka Shin Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut Tsuneyoshi Isao Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut Journal of Anesthesia Springer Japan 25/1(2011-02-01), 65-71 0913-8668 25:1<65 2011 25 540 https://doi.org/10.1007/s00540-010-1071-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00540-010-1071-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shirasaka Tetsuro Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut NATIONALLICENCE 700 1- Yonaha Tetsu Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut NATIONALLICENCE 700 1- Onizuka Shin Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut NATIONALLICENCE 700 1- Tsuneyoshi Isao Department of Anesthesiology and Intensive care, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, 889-1692, Miyazaki, Japan aut NATIONALLICENCE 773 0- Journal of Anesthesia Springer Japan 25/1(2011-02-01), 65-71 0913-8668 25:1<65 2011 25 540 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer