caa a22 4500 445373687 CHVBK 20180317142955.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00540-011-1142-3 doi (NATIONALLICENCE)springer-10.1007/s00540-011-1142-3 The antinociceptive effects of estradiol on adjuvant-induced hyperalgesia in rats involve activation of adrenergic and serotonergic systems [Elektronische Daten] [Kentaro Okuda, Hideo Iwasaka, Satoshi Hagiwara, Naozumi Takeshima, Junji Takatani, Tetsuya Uchino, Takayuki Noguchi] Purpose: Estradiol is a female hormone required for maintaining pregnancy and developing follicles in the ovary. Estradiol has been shown to perform a variety of physiological activities, including pain reduction. In this study, we tested the hypothesis that estradiol exerts antinociceptive effects in a rat model of inflammatory hyperalgesia. Methods: We established a subacute hyperalgesia model using plantar injection of Freund's complete adjuvant (FCA) in Sprague-Dawley rats. We administered estradiol every 24h, beginning 12h after FCA administration, and used the plantar test to determine its effect on hyperalgesia. To determine the mechanism of action of estradiol, we evaluated the role of the opioid antinociceptive system using naloxone and the role of the descending pain inhibitory system using the α-2-receptor antagonist yohimbine and the serotonin receptor antagonist methysergide. Results: Administration of FCA induced hyperalgesia, which was significantly reduced by estradiol treatment compared to controls. Moreover, this effect was not antagonized by naloxone, but was attenuated by α-2-receptor and serotonin-receptor antagonists. Conclusion: Estradiol is known to perform a variety of physiological functions. Our findings suggest that one such function is antinociception via an interaction with α-2 receptors and serotonin receptors. Japanese Society of Anesthesiologists, 2011 Estradiol nationallicence Freund's complete adjuvant (FCA) nationallicence Hyperalgesia nationallicence Naloxone nationallicence Descending pain inhibitory system nationallicence Okuda Kentaro Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Iwasaka Hideo Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Hagiwara Satoshi Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Takeshima Naozumi Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Takatani Junji Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Uchino Tetsuya Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Noguchi Takayuki Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut Journal of Anesthesia Springer Japan 25/3(2011-06-01), 392-397 0913-8668 25:3<392 2011 25 540 https://doi.org/10.1007/s00540-011-1142-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00540-011-1142-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okuda Kentaro Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Iwasaka Hideo Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Hagiwara Satoshi Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Takeshima Naozumi Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Takatani Junji Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Uchino Tetsuya Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 700 1- Noguchi Takayuki Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, 879-5593, Yufu, Oita, Japan aut NATIONALLICENCE 773 0- Journal of Anesthesia Springer Japan 25/3(2011-06-01), 392-397 0913-8668 25:3<392 2011 25 540 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer