caa a22 4500 445373695 CHVBK 20180317142955.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00540-011-1121-8 doi (NATIONALLICENCE)springer-10.1007/s00540-011-1121-8 Sevoflurane inhibits angiotensin II-induced Rho kinase-mediated contraction of vascular smooth muscle from spontaneously hypertensive rat [Elektronische Daten] [Nobuhiko Uematsu, Koji Ogawa, Yasuyuki Tokinaga, Kazuaki Tange, Yoshio Hatano] Purpose: Angiotensin II (Ang II)-induced vasoconstriction is mediated by changes in intracellular free Ca2+ concentration ([Ca2+] i ) and myofilament Ca2+ sensitivity. Protein kinase C- and Rho kinase-mediated signaling pathways are proposed for the regulation of the Ca2+ sensitization mechanisms. We have demonstrated that sevoflurane inhibits Rho kinase-mediated contraction of isolated rat aortic smooth muscle. A recent study demonstrated that Rho-kinase mediated Ca2+ sensitization was involved in the pathophysiology of hypertension. This study was designed to investigate the effects of sevoflurane on Ang II-induced Rho kinase-mediated vascular contraction in spontaneously hypertensive rats (SHR). Methods: The effects of sevoflurane on vasoconstriction, increase in [Ca2+] i , and membrane translocation of Rho kinase in response to Ang II were investigated in normotensive Wistar-Kyoto rats (WKY) and SHR, using an isometric force transducer, a fluorometer, and Western blotting, respectively. Results: The inhibitory effects of sevoflurane on Ang II (10−7M)-induced contraction were greater (P<0.05) in SHR than in WKY at the highest concentration of sevoflurane (5.1%). Y27632 (3×10−7M), a specific inhibitor of Rho kinase, inhibited the Ang II-induced contraction in SHR, but not in WKY. Sevoflurane did not affect the increases in [Ca2+] i in response to Ang II in either strain. Ang II stimulated Rho kinase activity in SHR, which was almost abolished by sevoflurane at a concentration of 5.1% (P<0.05). Conclusions: These findings suggest that the inhibition of the Ang II-induced contraction by sevoflurane in SHR may be, at least in part, due to the attenuation of the Rho kinase-mediated signaling pathway. Japanese Society of Anesthesiologists, 2011 Sevoflurane nationallicence Hypertension nationallicence Artery nationallicence Rho kinase nationallicence Uematsu Nobuhiko Department of Anesthesia, Japan Red Cross Society Wakayama Medical Center, Wakayama, Japan aut Ogawa Koji Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut Tokinaga Yasuyuki Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut Tange Kazuaki Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut Hatano Yoshio Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut Journal of Anesthesia Springer Japan 25/3(2011-06-01), 398-404 0913-8668 25:3<398 2011 25 540 https://doi.org/10.1007/s00540-011-1121-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00540-011-1121-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uematsu Nobuhiko Department of Anesthesia, Japan Red Cross Society Wakayama Medical Center, Wakayama, Japan aut NATIONALLICENCE 700 1- Ogawa Koji Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut NATIONALLICENCE 700 1- Tokinaga Yasuyuki Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut NATIONALLICENCE 700 1- Tange Kazuaki Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut NATIONALLICENCE 700 1- Hatano Yoshio Department of Anesthesiology, Wakayama Medical University, 811-1 Kimiidera, 641-0012, Wakayama, Japan aut NATIONALLICENCE 773 0- Journal of Anesthesia Springer Japan 25/3(2011-06-01), 398-404 0913-8668 25:3<398 2011 25 540 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer