caa a22 4500 445374039 CHVBK 20180317142956.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00540-011-1167-7 doi (NATIONALLICENCE)springer-10.1007/s00540-011-1167-7 Effects of sevoflurane on voltage-gated sodium channel Nav1.8, Nav1.7, and Nav1.4 expressed in Xenopus oocytes [Elektronische Daten] [Toru Yokoyama, Kouichiro Minami, Yuka Sudo, Takafumi Horishita, Junichi Ogata, Toshihiko Yanagita, Yasuhito Uezono] Sevoflurane is widely used as a volatile anesthetic in clinical practice. However, its mechanism is still unclear. Recently, it has been reported that voltage-gated sodium channels have important roles in anesthetic mechanisms. Much attention has been paid to the effects of sevoflurane on voltage-dependent sodium channels. To elucidate this, we examined the effects of sevoflurane on Nav 1.8, Nav 1.4, and Nav 1.7 expressed in Xenopus oocytes. The effects of sevoflurane on Nav 1.8, Nav 1.4, and Nav 1.7 sodium channels were studied by an electrophysiology method using whole-cell, two-electrode voltage-clamp techniques in Xenopus oocytes. Sevoflurane at 1.0mM inhibited the voltage-gated sodium channels Nav1.8, Nav1.4, and Nav1.7, but sevoflurane (0.5mM) had little effect. This inhibitory effect of 1mM sevoflurane was completely abolished by pretreatment with protein kinase C (PKC) inhibitor, bisindolylmaleimide I. Sevoflurane appears to have inhibitory effects on Nav1.8, Nav1.4, and Nav 1.7 by PKC pathways. However, these sodium channels might not be related to the clinical anesthetic effects of sevoflurane. Japanese Society of Anesthesiologists, 2011 Sevoflurane nationallicence Voltage-gated sodium channel nationallicence Xenopus oocytes nationallicence Yokoyama Toru Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 329-0483, Tochigi, Japan aut Minami Kouichiro Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 329-0483, Tochigi, Japan aut Sudo Yuka Cancer Pathophysiology Division, National Cancer Center Research Institute, Tokyo, Japan aut Horishita Takafumi Department of Anesthesiology, University of Occupational and Environmental Health, Kitakyushu, Japan aut Ogata Junichi Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 329-0483, Tochigi, Japan aut Yanagita Toshihiko Department of Pharmacology, University of Miyazaki, School of Medicine, Miyazaki, Japan aut Uezono Yasuhito Cancer Pathophysiology Division, National Cancer Center Research Institute, Tokyo, Japan aut Journal of Anesthesia Springer Japan 25/4(2011-08-01), 609-613 0913-8668 25:4<609 2011 25 540 https://doi.org/10.1007/s00540-011-1167-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00540-011-1167-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yokoyama Toru Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 329-0483, Tochigi, Japan aut NATIONALLICENCE 700 1- Minami Kouichiro Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 329-0483, Tochigi, Japan aut NATIONALLICENCE 700 1- Sudo Yuka Cancer Pathophysiology Division, National Cancer Center Research Institute, Tokyo, Japan aut NATIONALLICENCE 700 1- Horishita Takafumi Department of Anesthesiology, University of Occupational and Environmental Health, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Ogata Junichi Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 329-0483, Tochigi, Japan aut NATIONALLICENCE 700 1- Yanagita Toshihiko Department of Pharmacology, University of Miyazaki, School of Medicine, Miyazaki, Japan aut NATIONALLICENCE 700 1- Uezono Yasuhito Cancer Pathophysiology Division, National Cancer Center Research Institute, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Anesthesia Springer Japan 25/4(2011-08-01), 609-613 0913-8668 25:4<609 2011 25 540 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer