caa a22 4500 445374365 CHVBK 20180317142958.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00540-011-1184-6 doi (NATIONALLICENCE)springer-10.1007/s00540-011-1184-6 Direct assessment of the antioxidant properties of midazolam by electron spin resonance spectroscopy [Elektronische Daten] [Mitsuuru Hata, Kyo Kobayashi, Fumihiko Yoshino, Ayaka Yoshida, Shuta Sugiyama, Chihiro Miyamoto, Fumiaki Tokutomi, Yojiro Maehata, Satoko Wada-Takahashi, Shun-suke Takahashi, Tomoko Komatsu, Kazu-ichi Yoshida, Masaichi-Chang-il Lee] Some antioxidant anesthetics directly inhibit lipid peroxidation mediated via the generation of reactive oxygen species (ROS). To date, the scavenging effects of midazolam on ROS have not been directly assessed. We investigated the inhibitory effect of midazolam on ROS [hydroxyl radical (HO·) and superoxide (O2·-)] by in vitro X-band electron spin resonance with the spin-trapping agent 5,5-dimethyl-1-pyrroline-N-oxide. Our results indicated that HO· and O2·- were not affected by midazolam at clinically relevant concentrations, but were directly scavenged by midazolam at high concentrations (i.e., >4.6 and >1.5mM, respectively). Japanese Society of Anesthesiologists, 2011 Reactive oxygen species nationallicence Oxidative stress nationallicence Electron spin resonance (ESR) nationallicence Antioxidants nationallicence Midazolam nationallicence Hata Mitsuuru Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Kobayashi Kyo Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Yoshino Fumihiko Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Yoshida Ayaka Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Sugiyama Shuta Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Miyamoto Chihiro Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Tokutomi Fumiaki Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Maehata Yojiro Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Wada-Takahashi Satoko Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Takahashi Shun-suke Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Komatsu Tomoko Division of Dentistry for Special Patients, Department of Clinical Care Medicine, Kanagawa Dental College, Yokosuka, Kanagawa, Japan aut Yoshida Kazu-ichi Division of Anesthesiology, Department of Clinical Care Medicine, Kanagawa Dental College, Yokosuka, Kanagawa, Japan aut Lee Masaichi-Chang-il Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut Journal of Anesthesia Springer Japan 25/5(2011-10-01), 765-769 0913-8668 25:5<765 2011 25 540 https://doi.org/10.1007/s00540-011-1184-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00540-011-1184-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hata Mitsuuru Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kobayashi Kyo Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Yoshino Fumihiko Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Yoshida Ayaka Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sugiyama Shuta Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Miyamoto Chihiro Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Tokutomi Fumiaki Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Maehata Yojiro Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Wada-Takahashi Satoko Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Takahashi Shun-suke Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Komatsu Tomoko Division of Dentistry for Special Patients, Department of Clinical Care Medicine, Kanagawa Dental College, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Yoshida Kazu-ichi Division of Anesthesiology, Department of Clinical Care Medicine, Kanagawa Dental College, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Lee Masaichi-Chang-il Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 773 0- Journal of Anesthesia Springer Japan 25/5(2011-10-01), 765-769 0913-8668 25:5<765 2011 25 540 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer