caa a22 4500 44537604X CHVBK 20180317143002.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00702-010-0497-1 doi (NATIONALLICENCE)springer-10.1007/s00702-010-0497-1 NIR-labeled nanoparticles engineered for brain targeting: in vivo optical imaging application and fluorescent microscopy evidences [Elektronische Daten] [G. Tosi, L. Bondioli, B. Ruozi, L. Badiali, G. Severini, S. Biffi, A. De Vita, B. Bortot, D. Dolcetta, F. Forni, M. Vandelli] The presence of the blood-brain barrier (BBB) makes extremely difficult to develop efficacious strategies for targeting contrast agents and delivering drugs inside the Central Nervous System (CNS). To overcome this drawback, several kinds of CNS-targeted nanoparticles (NPs) have been developed. In particular, we proposed poly-lactide-co-glycolide (PLGA) NPs engineered with a simil-opioid glycopeptide (g7), which have already proved to be a promising tool for achieving a successful brain targeting after i.v. administration in rats. In order to obtain CNS-targeted NPs to use for in vivo imaging, we synthesized and administrated in mice PLGA NPs with double coverage: near-infrared (NIR) probe (DY-675) and g 7. The optical imaging clearly showed a brain localization of these novel NPs. Thus, a novel kind of NIR-labeled NPs were obtained, providing a new, in vivo detectable nanotechnology tool. Besides, the confocal and fluorescence microscopy evidences allowed to further confirm the ability of g 7 to promote not only the rat, but also the mouse BBB crossing. Springer-Verlag, 2010 Nanoparticles nationallicence NIR nationallicence Optical imaging nationallicence Confocal and fluorescence microscopy nationallicence Brain targeting nationallicence Tosi G. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut Bondioli L. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut Ruozi B. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut Badiali L. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut Severini G. Department of Molecular Medicine and Laboratory, Institute of Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy aut Biffi S. Optical Imaging Laboratory, CBM, Area Science Park, Trieste, Italy aut De Vita A. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut Bortot B. Department of Molecular Medicine and Laboratory, Institute of Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy aut Dolcetta D. Mauro Baschirotto Institute for Rare Diseases, B.I.R.D. Foundation, Vicenza, Italy aut Forni F. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut Vandelli M. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut Journal of Neural Transmission Springer Vienna 118/1(2011-01-01), 145-153 0300-9564 118:1<145 2011 118 702 https://doi.org/10.1007/s00702-010-0497-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0497-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tosi G. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Bondioli L. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Ruozi B. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Badiali L. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Severini G. Department of Molecular Medicine and Laboratory, Institute of Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy aut NATIONALLICENCE 700 1- Biffi S. Optical Imaging Laboratory, CBM, Area Science Park, Trieste, Italy aut NATIONALLICENCE 700 1- De Vita A. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Bortot B. Department of Molecular Medicine and Laboratory, Institute of Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy aut NATIONALLICENCE 700 1- Dolcetta D. Mauro Baschirotto Institute for Rare Diseases, B.I.R.D. Foundation, Vicenza, Italy aut NATIONALLICENCE 700 1- Forni F. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut NATIONALLICENCE 700 1- Vandelli M. Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/1(2011-01-01), 145-153 0300-9564 118:1<145 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer