caa a22 4500 445376163 CHVBK 20180317143003.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00702-010-0536-y doi (NATIONALLICENCE)springer-10.1007/s00702-010-0536-y Fluoxetine treatment induces EAAT2 expression in rat brain [Elektronische Daten] [M. Zink, S. Rapp, R. Donev, P. Gebicke-Haerter, J. Thome] Synaptic pathology and disturbed glutamatergic neurotransmission contribute to the neurobiology of depression. Reduced expression of glutamate transporters, most importantly excitatory amino acid transporter (EAAT2), was reported in human studies and animal models. We therefore assessed the effects of antidepressant treatment upon EAAT2 expression. Male Sprague-Dawley rats received daily intraperitoneal injections of the antidepressants desipramine (DES, N=7), fluoxetine (FLU, N=7), tranylcypromine (TRAN, N=5) or a saline control (CON, N=5) for a period of 14days. The expression of the major glial glutamate transporter EAAT2 was evaluated by semi-quantitative in situ hybridizations using a 35S-labeled cRNA probe. Treatment with FLU significantly induced EAAT2 expression in hippocampal and cortical regions in comparison with saline injections, while DES and TRAN-applications did not exert significant effects. It can be postulated that increased expression of EAAT2 may counterbalance the tonus of glutamatergic neurotransmission. Our findings are in concert with human post-mortem findings, valid animal models of depression, antidepressive effects of NMDA-antagonists, and the glutamatergic theory of depression. Further studies should examine the effects of antidepressant treatments upon EAAT2 expression in rodent models of depression to further elucidate the underlying molecular mechanisms. Springer-Verlag, 2010 Depression nationallicence EAAT nationallicence Glia nationallicence Glutamate nationallicence Learned helplessness nationallicence Transporter nationallicence Zink M. Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, P.O. Box 12 21 20, 68072, Mannheim, Germany aut Rapp S. Department of Cardiology, University Hospital Mannheim, University of Heidelberg, Heidelberg, Germany aut Donev R. Academic Unit of Psychiatry, Institute of Life Science, The School of Medicine, University of Wales Swansea, Swansea, UK aut Gebicke-Haerter P. Department of Psychopharmacology, Central Institute of Mental Health, University of Heidelberg, Mannheim, Germany aut Thome J. Academic Unit of Psychiatry, Institute of Life Science, The School of Medicine, University of Wales Swansea, Swansea, UK aut Journal of Neural Transmission Springer Vienna 118/6(2011-06-01), 849-855 0300-9564 118:6<849 2011 118 702 https://doi.org/10.1007/s00702-010-0536-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-010-0536-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zink M. Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, P.O. Box 12 21 20, 68072, Mannheim, Germany aut NATIONALLICENCE 700 1- Rapp S. Department of Cardiology, University Hospital Mannheim, University of Heidelberg, Heidelberg, Germany aut NATIONALLICENCE 700 1- Donev R. Academic Unit of Psychiatry, Institute of Life Science, The School of Medicine, University of Wales Swansea, Swansea, UK aut NATIONALLICENCE 700 1- Gebicke-Haerter P. Department of Psychopharmacology, Central Institute of Mental Health, University of Heidelberg, Mannheim, Germany aut NATIONALLICENCE 700 1- Thome J. Academic Unit of Psychiatry, Institute of Life Science, The School of Medicine, University of Wales Swansea, Swansea, UK aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/6(2011-06-01), 849-855 0300-9564 118:6<849 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer