caa a22 4500 445376430 CHVBK 20180317143004.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00702-011-0642-5 doi (NATIONALLICENCE)springer-10.1007/s00702-011-0642-5 Activation of cellular chemotactic responses to chemokines coupled with oxidation of plasma membrane proteins by lysyl oxidase [Elektronische Daten] [Héctor Lucero, Joni Mäki, Herbert Kagan] Lysyl oxidase (LOX) is a potent chemokine inducing the migration of varied cell types. Here we demonstrate that inhibition of cellular LOX activity by preincubation of vascular smooth muscle cells (VSMC) with β-aminopropionitrile (BAPN), the irreversible inhibitor of LOX activity, resulted in the marked suppression of the chemotactic response and sensitivity of these cells toward LOX and toward PDGF-BB. Plasma membranes purified from VSMC not previously exposed to BAPN contained a group of oxidized plasma membrane proteins, including the PDGF receptor, PDGFR-β. The oxidation of this receptor and other membrane proteins was largely prevented in cells preincubated with BAPN. Addition of purified LOX to BAPN-free cells, which had been previously exposed to BAPN, restored the profile of oxidized proteins towards that of control cells. The high affinity and capacity for the binding of PDGF-BB by cells was significantly diminished when compared with cells in which oxidation by LOX was prevented by BAPN. The chemotactic responses of LOX knock-out mouse embryonic fibroblasts mirrored those obtained with VSMC treated with BAPN. These novel findings suggest that LOX activity is essential to generate optimal chemotactic sensitivity of cells to chemoattractants by oxidizing specific cell surface proteins, such as PDGFR-β. Springer-Verlag, 2011 Chemotaxis nationallicence Lysyl oxidase nationallicence PDGF nationallicence Extracellular matrix nationallicence Lucero Héctor Department of Biochemistry, Boston University School of Medicine, 02118, Boston, MA, USA aut Mäki Joni Collagen Research Unit, Biocenter Oulu and Department of Medical, Biochemistry and Molecular Biology, University of Oulu, 90014, Oulu, Finland aut Kagan Herbert Department of Biochemistry, Boston University School of Medicine, 02118, Boston, MA, USA aut Journal of Neural Transmission Springer Vienna 118/7(2011-07-01), 1091-1099 0300-9564 118:7<1091 2011 118 702 https://doi.org/10.1007/s00702-011-0642-5 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00702-011-0642-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lucero Héctor Department of Biochemistry, Boston University School of Medicine, 02118, Boston, MA, USA aut NATIONALLICENCE 700 1- Mäki Joni Collagen Research Unit, Biocenter Oulu and Department of Medical, Biochemistry and Molecular Biology, University of Oulu, 90014, Oulu, Finland aut NATIONALLICENCE 700 1- Kagan Herbert Department of Biochemistry, Boston University School of Medicine, 02118, Boston, MA, USA aut NATIONALLICENCE 773 0- Journal of Neural Transmission Springer Vienna 118/7(2011-07-01), 1091-1099 0300-9564 118:7<1091 2011 118 702 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer